Direct stimulation of the guanine nucleotide exchange activity of p115 RoGEF by Gα13

Matthew J. Hart, Xuejun Jiang, Tohru Kozasa, William Roscoe, William D. Singer, Alfred G. Gilman, Paul C. Sternweis, Gideon Bollag

Research output: Contribution to journalReview articlepeer-review

658 Scopus citations

Abstract

Signaling pathways that link extracellular factors to activation of the monomeric guanosine triphosphatase (GTPase) Rho control cytoskeletal rearrangements and cell growth. Heterotrimeric guanine nucleotide-binding proteins (G proteins) participate in several of these pathways, although their mechanisms are unclear. The GTPase activities of two G protein α subunits, Gα12 and Gα13, are stimulated by the Rho guanine nucleotide exchange factor p115 RhoGEF. Activated Gα13 bound tightly to p115 RhoGEF and stimulated its capacity to catalyze nucleotide exchange on Rho. In contrast, activated Gα12 inhibited stimulation by Gα13. Thus, p115 RhoGEF can directly link heterotrimeric G protein α subunits to regulation of Rho.

Original languageEnglish (US)
Pages (from-to)2112-2114
Number of pages3
JournalScience
Volume280
Issue number5372
DOIs
StatePublished - Jun 26 1998
Externally publishedYes

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Direct stimulation of the guanine nucleotide exchange activity of p115 RoGEF by Gα<sub>13</sub>'. Together they form a unique fingerprint.

Cite this