Direct spectroscopic evidence for a high-spin Fe(IV) intermediate in tyrosine hydroxylase

Bekir E. Eser, Eric W. Barr, Patrick A. Frantom, Lana Saleh, J. Martin Bollinger, Carsten Krebs, Paul F Fitzpatrick

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

Tyrosine hydroxylase, a member of the aromatic amino acid hydroxylase family, uses a mononuclear Fe(II) and tetrahydropterin for hydroxylation of tyrosine to dihydroxyphenylalanine. Rapid-freeze quench Mössbauer spectroscopy has now provided direct evidence for the presence of an Fe(IV) intermediate in the reaction catalyzed by tyrosine hydroxylase. Rapid-quench techniques provide support for the kinetic competence of this species as the hydroxylating intermediate. This is the first direct evidence for a mononuclear Fe(IV) intermediate in an enzymatic aromatic hydroxylation reaction.

Original languageEnglish (US)
Pages (from-to)11334-11335
Number of pages2
JournalJournal of the American Chemical Society
Volume129
Issue number37
DOIs
StatePublished - Sep 19 2007
Externally publishedYes

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Hydroxylation
Tyrosine 3-Monooxygenase
Aromatic Amino Acids
Dihydroxyphenylalanine
Mixed Function Oxygenases
Carboxylic acids
Mental Competency
Tyrosine
Amino acids
Spectrum Analysis
Spectroscopy
Kinetics
tetrahydropterin

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Direct spectroscopic evidence for a high-spin Fe(IV) intermediate in tyrosine hydroxylase. / Eser, Bekir E.; Barr, Eric W.; Frantom, Patrick A.; Saleh, Lana; Bollinger, J. Martin; Krebs, Carsten; Fitzpatrick, Paul F.

In: Journal of the American Chemical Society, Vol. 129, No. 37, 19.09.2007, p. 11334-11335.

Research output: Contribution to journalArticle

Eser, Bekir E. ; Barr, Eric W. ; Frantom, Patrick A. ; Saleh, Lana ; Bollinger, J. Martin ; Krebs, Carsten ; Fitzpatrick, Paul F. / Direct spectroscopic evidence for a high-spin Fe(IV) intermediate in tyrosine hydroxylase. In: Journal of the American Chemical Society. 2007 ; Vol. 129, No. 37. pp. 11334-11335.
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AU - Bollinger, J. Martin

AU - Krebs, Carsten

AU - Fitzpatrick, Paul F

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