TY - JOUR
T1 - Direct PCR of Cryptococcus neoformans MATα and MATa pheromones to determine mating type, ploidy, and variety
T2 - A tool for epidemiological and molecular pathogenesis studies
AU - Chaturvedi, Sudha
AU - Rodeghier, Birgit
AU - Fan, Jinjiang
AU - Mcclelland, Carol M.
AU - Wickes, Brian L.
AU - Chaturvedi, Vishnu
PY - 2000
Y1 - 2000
N2 - Cryptococcus neoformans MATα and MATa pheromones were amplified by direct PCR. Nucleotide sequence analyses revealed unique restriction enzyme sites. Sixty strains were used to devise a restriction fragment length polymorphism typing scheme that yielded three variety-specific patterns. Additionally, pheromone-specific PCR allowed easier identification of diploid C. neoformans strains than flow cytometry.
AB - Cryptococcus neoformans MATα and MATa pheromones were amplified by direct PCR. Nucleotide sequence analyses revealed unique restriction enzyme sites. Sixty strains were used to devise a restriction fragment length polymorphism typing scheme that yielded three variety-specific patterns. Additionally, pheromone-specific PCR allowed easier identification of diploid C. neoformans strains than flow cytometry.
UR - http://www.scopus.com/inward/record.url?scp=0034016784&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034016784&partnerID=8YFLogxK
U2 - 10.1128/jcm.38.5.2007-2009.2000
DO - 10.1128/jcm.38.5.2007-2009.2000
M3 - Article
C2 - 10790143
AN - SCOPUS:0034016784
SN - 0095-1137
VL - 38
SP - 2007
EP - 2009
JO - Journal of clinical microbiology
JF - Journal of clinical microbiology
IS - 5
ER -