Cryptococcus neoformans MATα and MATa pheromones were amplified by direct PCR. Nucleotide sequence analyses revealed unique restriction enzyme sites. Sixty strains were used to devise a restriction fragment length polymorphism typing scheme that yielded three variety-specific patterns. Additionally, pheromone-specific PCR allowed easier identification of diploid C. neoformans strains than flow cytometry.
|Original language||English (US)|
|Number of pages||3|
|Journal||Journal of clinical microbiology|
|Publication status||Published - May 22 2000|
ASJC Scopus subject areas
- Microbiology (medical)