Direct PCR of Cryptococcus neoformans MATα and MATa pheromones to determine mating type, ploidy, and variety: A tool for epidemiological and molecular pathogenesis studies

Sudha Chaturvedi, Birgit Rodeghier, Jinjiang Fan, Carol M. Mcclelland, Brian L. Wickes, Vishnu Chaturvedi

Research output: Contribution to journalArticlepeer-review

61 Scopus citations

Abstract

Cryptococcus neoformans MATα and MATa pheromones were amplified by direct PCR. Nucleotide sequence analyses revealed unique restriction enzyme sites. Sixty strains were used to devise a restriction fragment length polymorphism typing scheme that yielded three variety-specific patterns. Additionally, pheromone-specific PCR allowed easier identification of diploid C. neoformans strains than flow cytometry.

Original languageEnglish (US)
Pages (from-to)2007-2009
Number of pages3
JournalJournal of clinical microbiology
Volume38
Issue number5
DOIs
StatePublished - 2000

ASJC Scopus subject areas

  • Microbiology (medical)

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