Direct PCR of Cryptococcus neoformans MATα and MATa pheromones to determine mating type, ploidy, and variety: A tool for epidemiological and molecular pathogenesis studies

Sudha Chaturvedi, Birgit Rodeghier, Jinjiang Fan, Carol M. Mcclelland, Brian L. Wickes, Vishnu Chaturvedi

Research output: Contribution to journalArticle

58 Scopus citations

Abstract

Cryptococcus neoformans MATα and MATa pheromones were amplified by direct PCR. Nucleotide sequence analyses revealed unique restriction enzyme sites. Sixty strains were used to devise a restriction fragment length polymorphism typing scheme that yielded three variety-specific patterns. Additionally, pheromone-specific PCR allowed easier identification of diploid C. neoformans strains than flow cytometry.

Original languageEnglish (US)
Pages (from-to)2007-2009
Number of pages3
JournalJournal of clinical microbiology
Volume38
Issue number5
Publication statusPublished - May 22 2000

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ASJC Scopus subject areas

  • Microbiology (medical)

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