TY - JOUR
T1 - Direct effect of intracanal medicaments on survival of stem cells of the apical papilla
AU - Ruparel, Nikita B.
AU - Teixeira, Fabricio B.
AU - Ferraz, Caio C.R.
AU - Diogenes, Anibal R
PY - 2012/10
Y1 - 2012/10
N2 - Introduction: Regenerative endodontic procedures are an alternative treatment for immature teeth with necrotic pulps. Typically, intracanal medicaments such as triple antibiotic paste (TAP) or double antibiotic paste (DAP) and calcium hydroxide (Ca[OH]2) are used for disinfection. However, their effect on human stem cells of the apical papilla (SCAPs) is unknown. We hypothesized that intracanal medicaments at high concentrations are toxic to SCAPs. To test this hypothesis, a cell culture assay was used. Methods: Briefly, SCAPs were cultured and subjected to either no drug treatment or various concentrations including TAP, DAP, modified TAP (ciprofloxacin, metronidazole and cefaclor), Augmentin (Champs Pharmacy, San Antonio, TX), or Ca(OH)2. Viable stem cells counts were obtained using an automated method of detecting trypan blue dye at 3 days after treatment. Results: All 4 antibiotics significantly reduced SCAP survival in a concentration-dependent fashion. Interestingly, Ca(OH)2 was conducive with SCAP survival at all concentrations. Conclusions: Collectively, our data show that high concentrations of antibiotics have a detrimental effect on SCAP survival, whereas lower concentrations as well as Ca(OH)2 at all tested concentrations are conducive with SCAP survival and proliferation. These studies highlight the clinically important point that intracanal medicaments must be used at concentrations that are bactericidal while having minimal effects on stem cell viability.
AB - Introduction: Regenerative endodontic procedures are an alternative treatment for immature teeth with necrotic pulps. Typically, intracanal medicaments such as triple antibiotic paste (TAP) or double antibiotic paste (DAP) and calcium hydroxide (Ca[OH]2) are used for disinfection. However, their effect on human stem cells of the apical papilla (SCAPs) is unknown. We hypothesized that intracanal medicaments at high concentrations are toxic to SCAPs. To test this hypothesis, a cell culture assay was used. Methods: Briefly, SCAPs were cultured and subjected to either no drug treatment or various concentrations including TAP, DAP, modified TAP (ciprofloxacin, metronidazole and cefaclor), Augmentin (Champs Pharmacy, San Antonio, TX), or Ca(OH)2. Viable stem cells counts were obtained using an automated method of detecting trypan blue dye at 3 days after treatment. Results: All 4 antibiotics significantly reduced SCAP survival in a concentration-dependent fashion. Interestingly, Ca(OH)2 was conducive with SCAP survival at all concentrations. Conclusions: Collectively, our data show that high concentrations of antibiotics have a detrimental effect on SCAP survival, whereas lower concentrations as well as Ca(OH)2 at all tested concentrations are conducive with SCAP survival and proliferation. These studies highlight the clinically important point that intracanal medicaments must be used at concentrations that are bactericidal while having minimal effects on stem cell viability.
KW - Apical papilla
KW - biocompatible
KW - calcium hydroxide
KW - dental stem cells
KW - double antibiotic
KW - human stem cells of the apical papilla
KW - regenerative
KW - triple antibiotic
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U2 - 10.1016/j.joen.2012.06.018
DO - 10.1016/j.joen.2012.06.018
M3 - Article
C2 - 22980180
AN - SCOPUS:84866355682
SN - 0099-2399
VL - 38
SP - 1372
EP - 1375
JO - Journal of endodontics
JF - Journal of endodontics
IS - 10
ER -