Direct current reduces plasma protein extravasation after partial- thickness burn injury in rats

Natalia P. Matylevich, Chi Sing Chu, Albert T. McManus, Arthur D. Mason, Basil A. Pruitt

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


Objective: To observe the effect of 40 μA direct current (DC) on plasma albumin extravasation after burn injury. Design, Materials, and Methods: Silver-nylon wound dressings were used as anodes (-) on anesthetized male Sprague-Dawley rats with 20% total body surface partial-thickness scald burns. Burned rats with no treatment, or treated with siver-nylon dressing without current, were used as controls. Quantitative analysis of fluorescein isothiocyanate (FITC)-albumin leakage and accumulation in the wound tissue was performed using confocal fluorescence microscopy. Measurements and Main Results: In controls, the rate of albumin leakage was maximal at 1 hour postburn (PB) and then decreased, but remained higher than normal for 48 hours PB. The accumulation of FITC-albumin was maximal 4 to 6 hours PB and substantial for 48 hours. When DC was applied, leakage was reduced by 30 to 45% and approached normal control rates by 8 hours PB. FITC-albumin concentration peaked 4 hours PB, was 18 to 48% less then in burned control, and approached the level observed in unburned control by 18 hours PB. Conclusions: DC has a beneficial effect in reducing plasma protein extravasation after burn injury.

Original languageEnglish (US)
Pages (from-to)424-429
Number of pages6
JournalJournal of Trauma - Injury, Infection and Critical Care
Issue number3
StatePublished - Sep 1996


  • Electric current
  • Partial-thickness burn
  • Protein extravasation
  • Silver-nylon

ASJC Scopus subject areas

  • Surgery
  • Critical Care and Intensive Care Medicine


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