Direct cloning of human malignant melanoma in soft agar culture

Daniel D. von Hoff, Barbara Forseth, Hans‐Robert ‐R Metelmann, Gary Harris, Sherri Rowan, Charles A. Coltman

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


An in vitro soft agar technique was used to culture human malignant melanoma cells from 61 solid tumors, 17 lymph nodes, 11 effusions, and four bone marrow specimens from 93 patients with malignant melanoma. Colonies grew in soft agar from 64 (69%) of the 93 specimens. Fifty‐five percent of the specimens cultured formed $30 colonies per 500,000 nucleated cells plated. Light microscopy, electron microscopy, tumor marker, and athymic nude mouse studies provided evidence the colonies were composed of malignant melanoma cells. Drug sensitivity studies utilizing the cloning technique showed similarities between in vitro results and the general clinical experience noted with the same drugs. The human tumor cloning system represents a new model for future basic biology and clinical studies of human malignant melanoma.

Original languageEnglish (US)
Pages (from-to)696-701
Number of pages6
Issue number4
StatePublished - Aug 15 1982
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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