Direct and sex-specific enhancement of bone formation and calcification by sex steroids in fetal mice long bone in vitro biochemical and morphometric study

Zvi Schwartz, W. A. Soskolne, T. Neubauer, M. Goldstein, S. Adi, A. Ornoy

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The study was carried out to examine the direct effect of the sex hormones 17β-estradiol (E2) and testosterone on the modeling of cultured fetal mouse long bones separated according to their sex. The culture system used allowed for the simultaneous assessment of bone growth, mineralization, and resorption on each bone. Bones from 16-day-old male and female mouse fetuses were cultured in BGJ medium, supplemented with either 10% fetal calf serum or 4 mg/ml BSA (serum-free medium) for 48 h. The bones were harvested, and their length; the length of their diaphyses; their hydroxyproline, calcium, and phosphorus contents; and their 45Ca release were measured. Histomorphometric analyses on midlongitudinal sections of bones from parallel experiments were also performed. The results indicate that in medium supplemented with 10% fetal calf serum, E2 had a dose-dependent stimulatory effect on bone formation and mineralization at 10-7 and 10-9 M, with no effect on bone resorption. This effect was specific to bones from female mice and to E2, since 17-α-estradiol had no effect. Testosterone had similar effects specific to bones from male mice, resulting in the stimulation of bone formation and mineralization at 10-7- and 10-9-M concentrations. These effects were absent when serum-free medium was used. E2 and testosterone had an anabolic effect on endochondral and periosteal bone formation and mineralization, but no effect on bone resorption. This effect is dependent on the presence of a serum factor(s).

Original languageEnglish (US)
Pages (from-to)1167-1174
Number of pages8
JournalEndocrinology
Volume129
Issue number3
StatePublished - Sep 1991
Externally publishedYes

Fingerprint

Osteogenesis
Steroids
Physiologic Calcification
Bone and Bones
Bone Resorption
Testosterone
Serum-Free Culture Media
Estradiol
Serum
Anabolic Agents
Diaphyses
Bone Development
Hydroxyproline
Gonadal Steroid Hormones
In Vitro Techniques
Phosphorus
Fetus
Calcium

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Schwartz, Z., Soskolne, W. A., Neubauer, T., Goldstein, M., Adi, S., & Ornoy, A. (1991). Direct and sex-specific enhancement of bone formation and calcification by sex steroids in fetal mice long bone in vitro biochemical and morphometric study. Endocrinology, 129(3), 1167-1174.

Direct and sex-specific enhancement of bone formation and calcification by sex steroids in fetal mice long bone in vitro biochemical and morphometric study. / Schwartz, Zvi; Soskolne, W. A.; Neubauer, T.; Goldstein, M.; Adi, S.; Ornoy, A.

In: Endocrinology, Vol. 129, No. 3, 09.1991, p. 1167-1174.

Research output: Contribution to journalArticle

Schwartz, Z, Soskolne, WA, Neubauer, T, Goldstein, M, Adi, S & Ornoy, A 1991, 'Direct and sex-specific enhancement of bone formation and calcification by sex steroids in fetal mice long bone in vitro biochemical and morphometric study', Endocrinology, vol. 129, no. 3, pp. 1167-1174.
Schwartz, Zvi ; Soskolne, W. A. ; Neubauer, T. ; Goldstein, M. ; Adi, S. ; Ornoy, A. / Direct and sex-specific enhancement of bone formation and calcification by sex steroids in fetal mice long bone in vitro biochemical and morphometric study. In: Endocrinology. 1991 ; Vol. 129, No. 3. pp. 1167-1174.
@article{7cf9f4fad3534b7eaf480428397924c9,
title = "Direct and sex-specific enhancement of bone formation and calcification by sex steroids in fetal mice long bone in vitro biochemical and morphometric study",
abstract = "The study was carried out to examine the direct effect of the sex hormones 17β-estradiol (E2) and testosterone on the modeling of cultured fetal mouse long bones separated according to their sex. The culture system used allowed for the simultaneous assessment of bone growth, mineralization, and resorption on each bone. Bones from 16-day-old male and female mouse fetuses were cultured in BGJ medium, supplemented with either 10{\%} fetal calf serum or 4 mg/ml BSA (serum-free medium) for 48 h. The bones were harvested, and their length; the length of their diaphyses; their hydroxyproline, calcium, and phosphorus contents; and their 45Ca release were measured. Histomorphometric analyses on midlongitudinal sections of bones from parallel experiments were also performed. The results indicate that in medium supplemented with 10{\%} fetal calf serum, E2 had a dose-dependent stimulatory effect on bone formation and mineralization at 10-7 and 10-9 M, with no effect on bone resorption. This effect was specific to bones from female mice and to E2, since 17-α-estradiol had no effect. Testosterone had similar effects specific to bones from male mice, resulting in the stimulation of bone formation and mineralization at 10-7- and 10-9-M concentrations. These effects were absent when serum-free medium was used. E2 and testosterone had an anabolic effect on endochondral and periosteal bone formation and mineralization, but no effect on bone resorption. This effect is dependent on the presence of a serum factor(s).",
author = "Zvi Schwartz and Soskolne, {W. A.} and T. Neubauer and M. Goldstein and S. Adi and A. Ornoy",
year = "1991",
month = "9",
language = "English (US)",
volume = "129",
pages = "1167--1174",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "3",

}

TY - JOUR

T1 - Direct and sex-specific enhancement of bone formation and calcification by sex steroids in fetal mice long bone in vitro biochemical and morphometric study

AU - Schwartz, Zvi

AU - Soskolne, W. A.

AU - Neubauer, T.

AU - Goldstein, M.

AU - Adi, S.

AU - Ornoy, A.

PY - 1991/9

Y1 - 1991/9

N2 - The study was carried out to examine the direct effect of the sex hormones 17β-estradiol (E2) and testosterone on the modeling of cultured fetal mouse long bones separated according to their sex. The culture system used allowed for the simultaneous assessment of bone growth, mineralization, and resorption on each bone. Bones from 16-day-old male and female mouse fetuses were cultured in BGJ medium, supplemented with either 10% fetal calf serum or 4 mg/ml BSA (serum-free medium) for 48 h. The bones were harvested, and their length; the length of their diaphyses; their hydroxyproline, calcium, and phosphorus contents; and their 45Ca release were measured. Histomorphometric analyses on midlongitudinal sections of bones from parallel experiments were also performed. The results indicate that in medium supplemented with 10% fetal calf serum, E2 had a dose-dependent stimulatory effect on bone formation and mineralization at 10-7 and 10-9 M, with no effect on bone resorption. This effect was specific to bones from female mice and to E2, since 17-α-estradiol had no effect. Testosterone had similar effects specific to bones from male mice, resulting in the stimulation of bone formation and mineralization at 10-7- and 10-9-M concentrations. These effects were absent when serum-free medium was used. E2 and testosterone had an anabolic effect on endochondral and periosteal bone formation and mineralization, but no effect on bone resorption. This effect is dependent on the presence of a serum factor(s).

AB - The study was carried out to examine the direct effect of the sex hormones 17β-estradiol (E2) and testosterone on the modeling of cultured fetal mouse long bones separated according to their sex. The culture system used allowed for the simultaneous assessment of bone growth, mineralization, and resorption on each bone. Bones from 16-day-old male and female mouse fetuses were cultured in BGJ medium, supplemented with either 10% fetal calf serum or 4 mg/ml BSA (serum-free medium) for 48 h. The bones were harvested, and their length; the length of their diaphyses; their hydroxyproline, calcium, and phosphorus contents; and their 45Ca release were measured. Histomorphometric analyses on midlongitudinal sections of bones from parallel experiments were also performed. The results indicate that in medium supplemented with 10% fetal calf serum, E2 had a dose-dependent stimulatory effect on bone formation and mineralization at 10-7 and 10-9 M, with no effect on bone resorption. This effect was specific to bones from female mice and to E2, since 17-α-estradiol had no effect. Testosterone had similar effects specific to bones from male mice, resulting in the stimulation of bone formation and mineralization at 10-7- and 10-9-M concentrations. These effects were absent when serum-free medium was used. E2 and testosterone had an anabolic effect on endochondral and periosteal bone formation and mineralization, but no effect on bone resorption. This effect is dependent on the presence of a serum factor(s).

UR - http://www.scopus.com/inward/record.url?scp=0026048415&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026048415&partnerID=8YFLogxK

M3 - Article

C2 - 1874163

AN - SCOPUS:0026048415

VL - 129

SP - 1167

EP - 1174

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 3

ER -