Diminished α-inhibin messenger ribonucleic acid in in vitro fertilization-embryo transfer poor responders reflects declining follicle reserve

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Abstract

Objectives: To quantitate and compare granulosa cell α-inhibin messenger RNA (mRNA) levels in IVF-ET poor and good responders and thereby learn how α-inhibin mRNA levels change in states of diminished ovarian responsiveness. Design: Ribonucleic acid analysis of stored luteinized granulosa cell samples. Setting: Academic tertiary care institution. Patients: Fifty-three women undergoing follicle aspiration for IVF-ET were studied. Patients were classified as poor responders (n = 16) or good responders (n = 37) according to their E2 concentration on the day of hCG; the E2 of poor responders was < 1,OO0 pg/mL (3,671 pmol/L) and that of good responders was ≥1,000 pg/mL (3,671 pmol/L). Main Outcome Measures: Messenger RNA levels were measured using dot blot RNA analysis. The following parameters were determined or derived: total mRNA levels, total α-inhibin mRNA levels, α-inhibin mRNA per follicle, and proportional α-inhibin mRNA as the ratio of α-inhibin mRNA:total mRNA. Results: Proportional α-inhibin mRNA and α-inhibin mRNA per follicle were not significantly different between poor responders and good responders. Total mRNA and total α-inhibin mRNA levels, however, were diminished significantly in poor responders. Conclusions: The observations that proportional α-inhibin mRNA and α-inhibin mRNA per follicle do not significantly change in poor responders, whereas total α-inhibin mRNA does, indicate that the decrease in total α-inhibin mRNA in poor responders reflects a decreased pool of total mRNA, likely because of a reduction in follicle number. These findings are in contrast to other recent reports that describe a change in granulosa cell function accompanying states of decreased ovarian responsiveness.

Original languageEnglish (US)
Pages (from-to)394-399
Number of pages6
JournalFertility and Sterility
Volume65
Issue number2
StatePublished - 1996

Fingerprint

Inhibins
Embryo Transfer
Fertilization in Vitro
RNA
Messenger RNA
Granulosa Cells

Keywords

  • α-inhibin
  • aging
  • follicle reserve
  • Granulosa cell function
  • IVF- ET
  • messenger RNA
  • poor responders

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

@article{64aabe99a27844898d45e61aad99eedb,
title = "Diminished α-inhibin messenger ribonucleic acid in in vitro fertilization-embryo transfer poor responders reflects declining follicle reserve",
abstract = "Objectives: To quantitate and compare granulosa cell α-inhibin messenger RNA (mRNA) levels in IVF-ET poor and good responders and thereby learn how α-inhibin mRNA levels change in states of diminished ovarian responsiveness. Design: Ribonucleic acid analysis of stored luteinized granulosa cell samples. Setting: Academic tertiary care institution. Patients: Fifty-three women undergoing follicle aspiration for IVF-ET were studied. Patients were classified as poor responders (n = 16) or good responders (n = 37) according to their E2 concentration on the day of hCG; the E2 of poor responders was < 1,OO0 pg/mL (3,671 pmol/L) and that of good responders was ≥1,000 pg/mL (3,671 pmol/L). Main Outcome Measures: Messenger RNA levels were measured using dot blot RNA analysis. The following parameters were determined or derived: total mRNA levels, total α-inhibin mRNA levels, α-inhibin mRNA per follicle, and proportional α-inhibin mRNA as the ratio of α-inhibin mRNA:total mRNA. Results: Proportional α-inhibin mRNA and α-inhibin mRNA per follicle were not significantly different between poor responders and good responders. Total mRNA and total α-inhibin mRNA levels, however, were diminished significantly in poor responders. Conclusions: The observations that proportional α-inhibin mRNA and α-inhibin mRNA per follicle do not significantly change in poor responders, whereas total α-inhibin mRNA does, indicate that the decrease in total α-inhibin mRNA in poor responders reflects a decreased pool of total mRNA, likely because of a reduction in follicle number. These findings are in contrast to other recent reports that describe a change in granulosa cell function accompanying states of decreased ovarian responsiveness.",
keywords = "α-inhibin, aging, follicle reserve, Granulosa cell function, IVF- ET, messenger RNA, poor responders",
author = "Burns, {W. N.} and Oktay, {K. H.} and Tekmal, {Rajeshwar R} and Nelson, {James F} and Schenken, {Robert S}",
year = "1996",
language = "English (US)",
volume = "65",
pages = "394--399",
journal = "Fertility and Sterility",
issn = "0015-0282",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - Diminished α-inhibin messenger ribonucleic acid in in vitro fertilization-embryo transfer poor responders reflects declining follicle reserve

AU - Burns, W. N.

AU - Oktay, K. H.

AU - Tekmal, Rajeshwar R

AU - Nelson, James F

AU - Schenken, Robert S

PY - 1996

Y1 - 1996

N2 - Objectives: To quantitate and compare granulosa cell α-inhibin messenger RNA (mRNA) levels in IVF-ET poor and good responders and thereby learn how α-inhibin mRNA levels change in states of diminished ovarian responsiveness. Design: Ribonucleic acid analysis of stored luteinized granulosa cell samples. Setting: Academic tertiary care institution. Patients: Fifty-three women undergoing follicle aspiration for IVF-ET were studied. Patients were classified as poor responders (n = 16) or good responders (n = 37) according to their E2 concentration on the day of hCG; the E2 of poor responders was < 1,OO0 pg/mL (3,671 pmol/L) and that of good responders was ≥1,000 pg/mL (3,671 pmol/L). Main Outcome Measures: Messenger RNA levels were measured using dot blot RNA analysis. The following parameters were determined or derived: total mRNA levels, total α-inhibin mRNA levels, α-inhibin mRNA per follicle, and proportional α-inhibin mRNA as the ratio of α-inhibin mRNA:total mRNA. Results: Proportional α-inhibin mRNA and α-inhibin mRNA per follicle were not significantly different between poor responders and good responders. Total mRNA and total α-inhibin mRNA levels, however, were diminished significantly in poor responders. Conclusions: The observations that proportional α-inhibin mRNA and α-inhibin mRNA per follicle do not significantly change in poor responders, whereas total α-inhibin mRNA does, indicate that the decrease in total α-inhibin mRNA in poor responders reflects a decreased pool of total mRNA, likely because of a reduction in follicle number. These findings are in contrast to other recent reports that describe a change in granulosa cell function accompanying states of decreased ovarian responsiveness.

AB - Objectives: To quantitate and compare granulosa cell α-inhibin messenger RNA (mRNA) levels in IVF-ET poor and good responders and thereby learn how α-inhibin mRNA levels change in states of diminished ovarian responsiveness. Design: Ribonucleic acid analysis of stored luteinized granulosa cell samples. Setting: Academic tertiary care institution. Patients: Fifty-three women undergoing follicle aspiration for IVF-ET were studied. Patients were classified as poor responders (n = 16) or good responders (n = 37) according to their E2 concentration on the day of hCG; the E2 of poor responders was < 1,OO0 pg/mL (3,671 pmol/L) and that of good responders was ≥1,000 pg/mL (3,671 pmol/L). Main Outcome Measures: Messenger RNA levels were measured using dot blot RNA analysis. The following parameters were determined or derived: total mRNA levels, total α-inhibin mRNA levels, α-inhibin mRNA per follicle, and proportional α-inhibin mRNA as the ratio of α-inhibin mRNA:total mRNA. Results: Proportional α-inhibin mRNA and α-inhibin mRNA per follicle were not significantly different between poor responders and good responders. Total mRNA and total α-inhibin mRNA levels, however, were diminished significantly in poor responders. Conclusions: The observations that proportional α-inhibin mRNA and α-inhibin mRNA per follicle do not significantly change in poor responders, whereas total α-inhibin mRNA does, indicate that the decrease in total α-inhibin mRNA in poor responders reflects a decreased pool of total mRNA, likely because of a reduction in follicle number. These findings are in contrast to other recent reports that describe a change in granulosa cell function accompanying states of decreased ovarian responsiveness.

KW - α-inhibin

KW - aging

KW - follicle reserve

KW - Granulosa cell function

KW - IVF- ET

KW - messenger RNA

KW - poor responders

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M3 - Article

C2 - 8566269

AN - SCOPUS:0030040653

VL - 65

SP - 394

EP - 399

JO - Fertility and Sterility

JF - Fertility and Sterility

SN - 0015-0282

IS - 2

ER -