Differentiation acceleration of healing of surgical incisions in the rabbit gastrointestinal tract by platelet-derived growth factor and transforming growth factor, type beta

T. A. Mustoe, A. Landes, D. T. Cromack, D. Mistry, A. Griffin, T. F. Deuel, G. F. Pierce

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

Anastomotic dehiscence is a major cause of morbidity and mortality in gastrointestinal surgery. A unique model system of a gastric incision was developed to test the potential of polypeptide growth factors to enhance wound healing. Paired, deep partial-thickness incisions to but not including the gastric mucosa were made. A single topical application of transforming growth factor, type beta 1 (TGF-β), platelet-derived growth factor, or control vehicle at the time of wounding was given. Wound breaking strength and detailed histologic analyses of wounds were evaluated as a function of time after wounding. TGF-β (0.1 to 2.0 μg/wound) demonstrated a bimodal, dose-dependent acceleration of wound breaking strength 7 days after gastric wounding. An approximate 4-day acceleration of gastric wound breaking strength by TGF-β (2 μg/wound) was seen at 7 and 11 days. Wounds treated with platelet-derived growth factor (10 μg/wound) displayed an increased cellular response but no enhancement of breaking strength at 7 and 11 days. These results demonstrate the ability of TGF-β to accelerate gastrointestinal tissue repair by topical application and suggest significant potential for the use of growth factors in enhancing repair of surgical wounds of the gastrointestinal tract.

Original languageEnglish (US)
Pages (from-to)324-330
Number of pages7
JournalSurgery
Volume108
Issue number2
StatePublished - 1990
Externally publishedYes

ASJC Scopus subject areas

  • Surgery

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