Differential synthesis of β-tubulin isotypes in gerbil nasal epithelia

Karen Woo, Heather C. Jensen-Smith, Richard F. Ludueña, Richard Hallworth

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Compartmentalization of β-tubulin isotypes within cells according to function was examined in gerbil olfactory and respiratory epithelia by using specific antibodies to four β-tubulin isotypes (βI, βII, βIII, and βIV). Isotype synthesis was cell-type-specific, but the localization of the isotypes was not compartmentalized. All four isotypes were found in the cilia, dendrites, somata, and axons of olfactory neurons. Only two isotypes (βI and βIV) were present in the cilia of nasal respiratory epithelial cells. The βIV isotype, thought to be an essential component of cilia, was present in olfactory neurons and respiratory epithelial cells, which are ciliated, but was not found in basal cells (the stem cells of olfactory sensory neurons, which have no cilia). Olfactory neurons therefore do not synthesize βIV-tubulin until they mature, when functioning cilia are also elaborated. The failure to observe compartmentalization of β-tubulin isotypes in olfactory neurons sheds new light on potential functions of the β-tubulin isotypes.

Original languageEnglish (US)
Pages (from-to)331-335
Number of pages5
JournalCell and Tissue Research
Volume309
Issue number2
DOIs
StatePublished - Sep 12 2002

Keywords

  • Cilia
  • Gerbil
  • Olfactory neuron
  • Respiratory epithelium
  • Tubulin isotypes
  • β-Tubulin

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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    Woo, K., Jensen-Smith, H. C., Ludueña, R. F., & Hallworth, R. (2002). Differential synthesis of β-tubulin isotypes in gerbil nasal epithelia. Cell and Tissue Research, 309(2), 331-335. https://doi.org/10.1007/s00441-002-0591-2