TY - JOUR
T1 - Differential regulation of the messenger RNA for three major senescence marker proteins in male rat liver
AU - Chatterjee, B.
AU - Nath, T. S.
AU - Roy, A. K.
N1 - Copyright:
Copyright 2004 Elsevier B.V., All rights reserved.
PY - 1981
Y1 - 1981
N2 - Changes in the mRNAs coding for specific hepatic proteins in male rats during aging were examined by in vitro translation of the liver mRNA in the rabbit reticulocyte lysate. Characterization of the [35S]methionine-labeled translation products by sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis followed by autoradiography showed major age-dependent changes in the hepatic concentrations of three mRNA species. The translation products of these three mRNAs were found to have Mr = 28,500, 26,300, and 19,500 and are called senescence marker proteins (SMP) 1, 2, and 3, respectively. On the basis of its immunochemical reactivity, SMP-3 (Mr = 19,500) is identified as α(2u)-globulin while the mRNAs for SMP-1 (Mr = 28,500) and SMP-2 (Mr = 26,300) code for two yet uncharacterized proteins. The liver of the prepubertal male rats was found to contain the mRNA for SMP-2 and showed almost complete absence of the mRNAs for SMP-1 and SMP-3. However, the mRNAs for both SMP-1 and SMP-3 were present in the postpubertal young adults while the mRNA for SMP-2 was absent. Finally, when the animals reached senescence, the mRNAs for SMP-1 and SMP-3 disappeared from the liver with the reappearance of the SMP-2 mRNA. Age-dependent regulation of the mRNAs for these three senescence marker proteins can serve as an important model for the study of differential gene expression during aging.
AB - Changes in the mRNAs coding for specific hepatic proteins in male rats during aging were examined by in vitro translation of the liver mRNA in the rabbit reticulocyte lysate. Characterization of the [35S]methionine-labeled translation products by sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis followed by autoradiography showed major age-dependent changes in the hepatic concentrations of three mRNA species. The translation products of these three mRNAs were found to have Mr = 28,500, 26,300, and 19,500 and are called senescence marker proteins (SMP) 1, 2, and 3, respectively. On the basis of its immunochemical reactivity, SMP-3 (Mr = 19,500) is identified as α(2u)-globulin while the mRNAs for SMP-1 (Mr = 28,500) and SMP-2 (Mr = 26,300) code for two yet uncharacterized proteins. The liver of the prepubertal male rats was found to contain the mRNA for SMP-2 and showed almost complete absence of the mRNAs for SMP-1 and SMP-3. However, the mRNAs for both SMP-1 and SMP-3 were present in the postpubertal young adults while the mRNA for SMP-2 was absent. Finally, when the animals reached senescence, the mRNAs for SMP-1 and SMP-3 disappeared from the liver with the reappearance of the SMP-2 mRNA. Age-dependent regulation of the mRNAs for these three senescence marker proteins can serve as an important model for the study of differential gene expression during aging.
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M3 - Article
C2 - 7240182
AN - SCOPUS:0019860295
SN - 0021-9258
VL - 256
SP - 5939
EP - 5941
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 12
ER -