Differential regulation of the anti-crossover and replication fork regression activities of mph1 by mte1

Xiaoyu Xue, Alma Papusha, Koyi Choi, Jaclyn N. Bonner, Sandeep Kumar, Hengyao Niu, Hardeep Kaur, Xiao Feng Zheng, Roberto A. Donnianni, Lucy Lu, Michael Lichten, Xiaolan Zhao, Grzegorz Ira, Patrick Sung

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

We identified Mte1 (Mph1-associated telomere maintenance protein 1) as a multifunctional regulator of Saccharomyces cerevisiae Mph1, a member of the FANCM family of DNA motor proteins important for DNA replication fork repair and crossover suppression during homologous recombination. We show that Mte1 interacts with Mph1 andDNAspecies that resemble aDNAreplication fork and theDloop formed during recombination. Biochemically, Mte1 stimulates Mph1-mediated DNA replication fork regression and branch migration in a model substrate. Consistent with this activity, genetic analysis reveals that Mte1 functions with Mph1 and the associated MHF complex in replication fork repair. Surprisingly, Mte1 antagonizes the D-loop-dissociative activity of Mph1–MHF and exerts a procrossover role in mitotic recombination. We further show that the influence of Mte1 on Mph1 activities requires its binding to Mph1 and DNA. Thus, Mte1 differentially regulates Mph1 activities to achieve distinct outcomes in recombination and replication fork repair.

Original languageEnglish (US)
Pages (from-to)687-699
Number of pages13
JournalGenes and Development
Volume30
Issue number6
DOIs
StatePublished - Mar 15 2016
Externally publishedYes

Keywords

  • Crossover formation
  • Genome replication
  • Homologous recombination
  • MHF
  • Mph1
  • Mte1

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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  • Cite this

    Xue, X., Papusha, A., Choi, K., Bonner, J. N., Kumar, S., Niu, H., Kaur, H., Zheng, X. F., Donnianni, R. A., Lu, L., Lichten, M., Zhao, X., Ira, G., & Sung, P. (2016). Differential regulation of the anti-crossover and replication fork regression activities of mph1 by mte1. Genes and Development, 30(6), 687-699. https://doi.org/10.1101/gad.276139.115