@article{66331baf0bf946619ebcb8345af8dfa4,
title = "Differential induction of NPR1 during defense responses in Brassica juncea",
abstract = "We have cloned an NPR1-homolog from mustard and found that NPR1 transcript level was elevated in mustard leaves upon treatment with salicylic acid (SA) and during infection with biotrophic fungal pathogen Erysiphe cruciferarum. Conversely, necrotrophic fungal pathogen Alternaria brassicicola, which caused severe tissue necrosis had apparently no effect on BjNPR1 expression. During these challenges the differential activation of SA and jasmonic acid responsive defense pathways was detected using marker genes PR1, defensin and AOC. The BjNPR1 protein was detected as high molecular weight oligomeric and reduced monomeric forms. We identified a monopartite NLS at the C-terminus of BjNPR1 amino acid sequence and transient transformation with NPR1-GFP fusion construct revealed nuclear translocation of BjNPR1 protein upon induction with SA.",
keywords = "Alternaria brassicicola, Erysiphe cruciferarum, Mustard, NPR1, SAR, Salicylic acid",
author = "Gargi Meur and Madhusudhan Budatha and {Dutta Gupta}, Aparna and Shyam Prakash and Kirti, {P. B.}",
note = "Funding Information: Andhra Pradesh-Netherlands/Biotechnology program, administered by the Institute of Public Enterprise, Osmania University Campus, Hyderabad, India has supported this work. Gargi Meur and Madhusudhan Budatha were supported by research fellowships from Council for Scientific and Industrial Research (CSIR), India. We thank Prof. C. Wasternack (Leibniz Institute of Plant Biochemistry, Weinberg, Germany) for AOC clone, Dr. K. Ulaganathan (Osmania University, Hyderabad, India) for a synthetic RsAFP2 gene, Dr. X. Dong (Duke University, NC, USA) for providing the pBI1.4t-NPR1, Dr. Irute Meskiene (University of Vienna, Inst. Microbiology and Genetics, Austria) for 2×35S-TL-GFP plasmid and Dr. S.R. Bhat (NRCPB, Indian Agricultural Research Institute, NewDelhi, India) for all the Brassica lines. We would like to acknowledge kind help from Dr. M. Maheshwari (CRIDA, Hyderabad, India) for helping with the biolistic transformations. Other Assistance Programs like UGC-SAP, COSIST and DST-FIST to the School of Life Sciences have been gratefully acknowledged.",
year = "2006",
month = apr,
doi = "10.1016/j.pmpp.2006.09.003",
language = "English (US)",
volume = "68",
pages = "128--137",
journal = "Physiological and Molecular Plant Pathology",
issn = "0885-5765",
publisher = "Academic Press Inc.",
number = "4-6",
}