Differential induction of NPR1 during defense responses in Brassica juncea

Gargi Meur; Madhusudhan Budatha; Aparna Dutta Gupta; Shyam Prakash; P. B. Kirti

doi:10.1016/j.pmpp.2006.09.003

Differential induction of NPR1 during defense responses in Brassica juncea

Gargi Meur, Madhusudhan Budatha, Aparna Dutta Gupta, Shyam Prakash, P. B. Kirti

Research output: Contribution to journal › Article

20 Scopus citations

Abstract

We have cloned an NPR1-homolog from mustard and found that NPR1 transcript level was elevated in mustard leaves upon treatment with salicylic acid (SA) and during infection with biotrophic fungal pathogen Erysiphe cruciferarum. Conversely, necrotrophic fungal pathogen Alternaria brassicicola, which caused severe tissue necrosis had apparently no effect on BjNPR1 expression. During these challenges the differential activation of SA and jasmonic acid responsive defense pathways was detected using marker genes PR1, defensin and AOC. The BjNPR1 protein was detected as high molecular weight oligomeric and reduced monomeric forms. We identified a monopartite NLS at the C-terminus of BjNPR1 amino acid sequence and transient transformation with NPR1-GFP fusion construct revealed nuclear translocation of BjNPR1 protein upon induction with SA.

Original language	English (US)
Pages (from-to)	128-137
Number of pages	10
Journal	Physiological and Molecular Plant Pathology
Volume	68
Issue number	4-6
DOIs	https://doi.org/10.1016/j.pmpp.2006.09.003
State	Published - Apr 1 2006
Externally published	Yes

Keywords

Alternaria brassicicola
Erysiphe cruciferarum
Mustard
NPR1
Salicylic acid
SAR

ASJC Scopus subject areas

Genetics
Plant Science

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Meur, G., Budatha, M., Dutta Gupta, A., Prakash, S., & Kirti, P. B. (2006). Differential induction of NPR1 during defense responses in Brassica juncea. Physiological and Molecular Plant Pathology, 68(4-6), 128-137. https://doi.org/10.1016/j.pmpp.2006.09.003

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title = "Differential induction of NPR1 during defense responses in Brassica juncea",

abstract = "We have cloned an NPR1-homolog from mustard and found that NPR1 transcript level was elevated in mustard leaves upon treatment with salicylic acid (SA) and during infection with biotrophic fungal pathogen Erysiphe cruciferarum. Conversely, necrotrophic fungal pathogen Alternaria brassicicola, which caused severe tissue necrosis had apparently no effect on BjNPR1 expression. During these challenges the differential activation of SA and jasmonic acid responsive defense pathways was detected using marker genes PR1, defensin and AOC. The BjNPR1 protein was detected as high molecular weight oligomeric and reduced monomeric forms. We identified a monopartite NLS at the C-terminus of BjNPR1 amino acid sequence and transient transformation with NPR1-GFP fusion construct revealed nuclear translocation of BjNPR1 protein upon induction with SA.",

keywords = "Alternaria brassicicola, Erysiphe cruciferarum, Mustard, NPR1, Salicylic acid, SAR",

author = "Gargi Meur and Madhusudhan Budatha and {Dutta Gupta}, Aparna and Shyam Prakash and Kirti, {P. B.}",

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AU - Meur, Gargi

AU - Budatha, Madhusudhan

AU - Dutta Gupta, Aparna

AU - Prakash, Shyam

AU - Kirti, P. B.

PY - 2006/4/1

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N2 - We have cloned an NPR1-homolog from mustard and found that NPR1 transcript level was elevated in mustard leaves upon treatment with salicylic acid (SA) and during infection with biotrophic fungal pathogen Erysiphe cruciferarum. Conversely, necrotrophic fungal pathogen Alternaria brassicicola, which caused severe tissue necrosis had apparently no effect on BjNPR1 expression. During these challenges the differential activation of SA and jasmonic acid responsive defense pathways was detected using marker genes PR1, defensin and AOC. The BjNPR1 protein was detected as high molecular weight oligomeric and reduced monomeric forms. We identified a monopartite NLS at the C-terminus of BjNPR1 amino acid sequence and transient transformation with NPR1-GFP fusion construct revealed nuclear translocation of BjNPR1 protein upon induction with SA.

AB - We have cloned an NPR1-homolog from mustard and found that NPR1 transcript level was elevated in mustard leaves upon treatment with salicylic acid (SA) and during infection with biotrophic fungal pathogen Erysiphe cruciferarum. Conversely, necrotrophic fungal pathogen Alternaria brassicicola, which caused severe tissue necrosis had apparently no effect on BjNPR1 expression. During these challenges the differential activation of SA and jasmonic acid responsive defense pathways was detected using marker genes PR1, defensin and AOC. The BjNPR1 protein was detected as high molecular weight oligomeric and reduced monomeric forms. We identified a monopartite NLS at the C-terminus of BjNPR1 amino acid sequence and transient transformation with NPR1-GFP fusion construct revealed nuclear translocation of BjNPR1 protein upon induction with SA.

KW - Alternaria brassicicola

KW - Erysiphe cruciferarum

KW - Mustard

KW - NPR1

KW - Salicylic acid

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