Differential expression of thrombospondin and cellular fibronectin during remodeling in proliferative glomerulonephritis

Jeffrey L. Barnes, Ronda J. Mitchell, John J. Kanalas, Veronique L. Barnes

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Thrombospondin-1 (TSP-1) and an alternatively spliced fibronectin (Fn)- EIIIA isoform are adhesive proteins associated with embryogenesis and tissue remodeling. We compared, by immunohistochemistry and in situ hybridization, the course of TSP-1 and Fn-EIIIA expression in a model of glomerulonephritis induced by Habu snake venom (HV) and characterized by mesangial cell migration, proliferation, and extracellular matrix (ECM) synthesis. At 24 hr after HV, TSP-1 and Fn-EIIIA proteins localized in the central aspects of lesions associated with platelets and macrophages and at the margins of lesions coinciding with mesangial cell migration (determined by Thy-1 staining). Mesangial cells at this time expressed TSP-1 but not Fn-EIIIA mRNA. TSP-1 protein and mRNA peaked in lesions at 48 hr and were associated with cell proliferation (determined by PCNA, α-smooth muscle actin phenotype, and expression of β-PDGF receptor mRNA). TSP-1 expression declined at 72 hr when expression of ECM synthesis peaked, as determined by increased expression of collagen Type IV, laminin, and TGF-β1 protein and mRNA. Mesangial cell expression of Fn-EIIIA was first observed at 48 hr and was most abundant at 72 hr after HV. Therefore, platelet- and macrophage- derived Fn-EIIIA and TSP-1 in early lesions are associated with mesangial cell migration. Mesangial cell upregulation of TSP-1 is associated with migration and proliferation but not maximal ECM accumulation, whereas mesangial cell expression of Fn-EIIIA is associated with proliferation and ECM accumulation. These results suggest distinctive temporal and spatial roles for TSP-1 and Fn-EIIIA in remodeling during glomerular disease.

Original languageEnglish (US)
Pages (from-to)533-543
Number of pages11
JournalJournal of Histochemistry and Cytochemistry
Volume47
Issue number4
StatePublished - Apr 1999

Fingerprint

Thrombospondin 1
Thrombospondins
Glomerulonephritis
Fibronectins
Mesangial Cells
Trimeresurus
Snake Venoms
Extracellular Matrix
Cell Movement
Messenger RNA
Blood Platelets
Macrophages
Cell Proliferation
Platelet-Derived Growth Factor Receptors
Proteins
Collagen Type IV
Proliferating Cell Nuclear Antigen
Adhesives
Embryonic Development
In Situ Hybridization

Keywords

  • Alternatively spliced fibronectin
  • Extracellular matrix
  • Mesangial cell
  • Migration
  • Proliferation
  • Thrombospondin

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Differential expression of thrombospondin and cellular fibronectin during remodeling in proliferative glomerulonephritis. / Barnes, Jeffrey L.; Mitchell, Ronda J.; Kanalas, John J.; Barnes, Veronique L.

In: Journal of Histochemistry and Cytochemistry, Vol. 47, No. 4, 04.1999, p. 533-543.

Research output: Contribution to journalArticle

Barnes, Jeffrey L. ; Mitchell, Ronda J. ; Kanalas, John J. ; Barnes, Veronique L. / Differential expression of thrombospondin and cellular fibronectin during remodeling in proliferative glomerulonephritis. In: Journal of Histochemistry and Cytochemistry. 1999 ; Vol. 47, No. 4. pp. 533-543.
@article{8554b04cc88d439eb715e0c0b26c37ab,
title = "Differential expression of thrombospondin and cellular fibronectin during remodeling in proliferative glomerulonephritis",
abstract = "Thrombospondin-1 (TSP-1) and an alternatively spliced fibronectin (Fn)- EIIIA isoform are adhesive proteins associated with embryogenesis and tissue remodeling. We compared, by immunohistochemistry and in situ hybridization, the course of TSP-1 and Fn-EIIIA expression in a model of glomerulonephritis induced by Habu snake venom (HV) and characterized by mesangial cell migration, proliferation, and extracellular matrix (ECM) synthesis. At 24 hr after HV, TSP-1 and Fn-EIIIA proteins localized in the central aspects of lesions associated with platelets and macrophages and at the margins of lesions coinciding with mesangial cell migration (determined by Thy-1 staining). Mesangial cells at this time expressed TSP-1 but not Fn-EIIIA mRNA. TSP-1 protein and mRNA peaked in lesions at 48 hr and were associated with cell proliferation (determined by PCNA, α-smooth muscle actin phenotype, and expression of β-PDGF receptor mRNA). TSP-1 expression declined at 72 hr when expression of ECM synthesis peaked, as determined by increased expression of collagen Type IV, laminin, and TGF-β1 protein and mRNA. Mesangial cell expression of Fn-EIIIA was first observed at 48 hr and was most abundant at 72 hr after HV. Therefore, platelet- and macrophage- derived Fn-EIIIA and TSP-1 in early lesions are associated with mesangial cell migration. Mesangial cell upregulation of TSP-1 is associated with migration and proliferation but not maximal ECM accumulation, whereas mesangial cell expression of Fn-EIIIA is associated with proliferation and ECM accumulation. These results suggest distinctive temporal and spatial roles for TSP-1 and Fn-EIIIA in remodeling during glomerular disease.",
keywords = "Alternatively spliced fibronectin, Extracellular matrix, Mesangial cell, Migration, Proliferation, Thrombospondin",
author = "Barnes, {Jeffrey L.} and Mitchell, {Ronda J.} and Kanalas, {John J.} and Barnes, {Veronique L.}",
year = "1999",
month = "4",
language = "English (US)",
volume = "47",
pages = "533--543",
journal = "Journal of Histochemistry and Cytochemistry",
issn = "0022-1554",
publisher = "Histochemical Society Inc.",
number = "4",

}

TY - JOUR

T1 - Differential expression of thrombospondin and cellular fibronectin during remodeling in proliferative glomerulonephritis

AU - Barnes, Jeffrey L.

AU - Mitchell, Ronda J.

AU - Kanalas, John J.

AU - Barnes, Veronique L.

PY - 1999/4

Y1 - 1999/4

N2 - Thrombospondin-1 (TSP-1) and an alternatively spliced fibronectin (Fn)- EIIIA isoform are adhesive proteins associated with embryogenesis and tissue remodeling. We compared, by immunohistochemistry and in situ hybridization, the course of TSP-1 and Fn-EIIIA expression in a model of glomerulonephritis induced by Habu snake venom (HV) and characterized by mesangial cell migration, proliferation, and extracellular matrix (ECM) synthesis. At 24 hr after HV, TSP-1 and Fn-EIIIA proteins localized in the central aspects of lesions associated with platelets and macrophages and at the margins of lesions coinciding with mesangial cell migration (determined by Thy-1 staining). Mesangial cells at this time expressed TSP-1 but not Fn-EIIIA mRNA. TSP-1 protein and mRNA peaked in lesions at 48 hr and were associated with cell proliferation (determined by PCNA, α-smooth muscle actin phenotype, and expression of β-PDGF receptor mRNA). TSP-1 expression declined at 72 hr when expression of ECM synthesis peaked, as determined by increased expression of collagen Type IV, laminin, and TGF-β1 protein and mRNA. Mesangial cell expression of Fn-EIIIA was first observed at 48 hr and was most abundant at 72 hr after HV. Therefore, platelet- and macrophage- derived Fn-EIIIA and TSP-1 in early lesions are associated with mesangial cell migration. Mesangial cell upregulation of TSP-1 is associated with migration and proliferation but not maximal ECM accumulation, whereas mesangial cell expression of Fn-EIIIA is associated with proliferation and ECM accumulation. These results suggest distinctive temporal and spatial roles for TSP-1 and Fn-EIIIA in remodeling during glomerular disease.

AB - Thrombospondin-1 (TSP-1) and an alternatively spliced fibronectin (Fn)- EIIIA isoform are adhesive proteins associated with embryogenesis and tissue remodeling. We compared, by immunohistochemistry and in situ hybridization, the course of TSP-1 and Fn-EIIIA expression in a model of glomerulonephritis induced by Habu snake venom (HV) and characterized by mesangial cell migration, proliferation, and extracellular matrix (ECM) synthesis. At 24 hr after HV, TSP-1 and Fn-EIIIA proteins localized in the central aspects of lesions associated with platelets and macrophages and at the margins of lesions coinciding with mesangial cell migration (determined by Thy-1 staining). Mesangial cells at this time expressed TSP-1 but not Fn-EIIIA mRNA. TSP-1 protein and mRNA peaked in lesions at 48 hr and were associated with cell proliferation (determined by PCNA, α-smooth muscle actin phenotype, and expression of β-PDGF receptor mRNA). TSP-1 expression declined at 72 hr when expression of ECM synthesis peaked, as determined by increased expression of collagen Type IV, laminin, and TGF-β1 protein and mRNA. Mesangial cell expression of Fn-EIIIA was first observed at 48 hr and was most abundant at 72 hr after HV. Therefore, platelet- and macrophage- derived Fn-EIIIA and TSP-1 in early lesions are associated with mesangial cell migration. Mesangial cell upregulation of TSP-1 is associated with migration and proliferation but not maximal ECM accumulation, whereas mesangial cell expression of Fn-EIIIA is associated with proliferation and ECM accumulation. These results suggest distinctive temporal and spatial roles for TSP-1 and Fn-EIIIA in remodeling during glomerular disease.

KW - Alternatively spliced fibronectin

KW - Extracellular matrix

KW - Mesangial cell

KW - Migration

KW - Proliferation

KW - Thrombospondin

UR - http://www.scopus.com/inward/record.url?scp=0032959828&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032959828&partnerID=8YFLogxK

M3 - Article

C2 - 10082755

AN - SCOPUS:0032959828

VL - 47

SP - 533

EP - 543

JO - Journal of Histochemistry and Cytochemistry

JF - Journal of Histochemistry and Cytochemistry

SN - 0022-1554

IS - 4

ER -