Differential expression of non-canonical wnts during osteoblast differentiation on 3-dimensional additively manufactured constructs

Michael B. Berger, Kyla Bosh, Thomas W. Jacobs, Barbara D. Boyan, Zvi Schwartz

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Orthopaedic and dental implant retention rates decrease in patients with one or a combination of risk factors such as age, diabetes or smoking status, or trauma resulting in reduced bone density. Therefore, there is a growing clinical need to create implants with material properties that promote accelerated osseointegration and reduced recovery time in compromised bone. One method to enhance this predictability is to increase the degree of osseointegration through additively manufactured (AM) implants possessing 3-dimensional (3D) macro-porosity. Osseointegration is the structural and functional connection of a titanium (Ti) implant with natural bone and AM 3D implants have been shown to enhance this functional connection with native tissue through increased mechanical stability and stimulation of bone in-growth. Osteogenesis around and into the macro-porosity of a placed implant requires the migration of mesenchymal stem cells (MSCs) and MSC differentiation into osteoblasts (OBs) to secrete and mineralize organic matrix. Our lab and others have shown Ti surfaces possessing microtextured topographies direct MSC differentiation into OBs without the addition of exogenous media supplements via a process involving non-canonical Wnt signaling. Therefore, in the present study, we examine the signaling mechanisms during the differentiation of pre-osteoblasts on 3D AM constructs in vitro.

Original languageEnglish (US)
Title of host publicationSociety for Biomaterials Annual Meeting and Exposition 2019
Subtitle of host publicationThe Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting
PublisherSociety for Biomaterials
Number of pages1
ISBN (Electronic)9781510883901
StatePublished - Jan 1 2019
Event42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Seattle, United States
Duration: Apr 3 2019Apr 6 2019

Publication series

NameTransactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
Volume40
ISSN (Print)1526-7547

Conference

Conference42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
CountryUnited States
CitySeattle
Period4/3/194/6/19

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ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Biotechnology
  • Biomaterials
  • Materials Chemistry

Cite this

Berger, M. B., Bosh, K., Jacobs, T. W., Boyan, B. D., & Schwartz, Z. (2019). Differential expression of non-canonical wnts during osteoblast differentiation on 3-dimensional additively manufactured constructs. In Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium; Vol. 40). Society for Biomaterials.