Differential expression of intestinal genes in opossums with high and low responses to dietary cholesterol

Jeannie Chan, Rampratap S. Kushwaha, Jane F. Vandeberg, Jelica Gluhak-Heinrich, John L. Vandeberg

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

High and low responding opossums (Monodelphis domestica) differ in their plasma very low density lipoprotein and low density lipoprotein (VLDL+LDL) cholesterol concentrations when they consume a high cholesterol diet, which is due in part to absorption of a higher percentage of dietary cholesterol in high responders. We compared the expression of a set of genes that influence cholesterol absorption in high and low responders fed a basal or a high cholesterol and low fat (HCLF) diet. Up-regulation of the ABCG5, ABCG8, and IBABP genes by the HCLF diet in high and low responders may reduce cholesterol absorption to maintain cholesterol homeostasis. Differences in expression of the phospholipase genes (PLA2 and PLB) and phospholipase activity were associated with differences in cholesterol absorption when opossums were fed cholesterol-enriched diets. Higher PLA2 and PLB mRNA levels and higher phospholipase activity may increase cholesterol absorption in high responders by enhancing the release of cholesterol from bile salt micelles for uptake by intestinal cells.

Original languageEnglish (US)
Article number415075
JournalJournal of Nutrition and Metabolism
Volume2010
DOIs
StatePublished - 2010

Fingerprint

Dietary Cholesterol
Opossums
opossums
Cholesterol
cholesterol
Gene Expression
genes
Phospholipases
Fat-Restricted Diet
low fat diet
High Fat Diet
Monodelphis
Monodelphis domestica
Diet
VLDL Cholesterol
bile salts
VLDL Lipoproteins
very low density lipoprotein
Micelles
micelles

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Food Science
  • Nutrition and Dietetics

Cite this

Differential expression of intestinal genes in opossums with high and low responses to dietary cholesterol. / Chan, Jeannie; Kushwaha, Rampratap S.; Vandeberg, Jane F.; Gluhak-Heinrich, Jelica; Vandeberg, John L.

In: Journal of Nutrition and Metabolism, Vol. 2010, 415075, 2010.

Research output: Contribution to journalArticle

Chan, Jeannie ; Kushwaha, Rampratap S. ; Vandeberg, Jane F. ; Gluhak-Heinrich, Jelica ; Vandeberg, John L. / Differential expression of intestinal genes in opossums with high and low responses to dietary cholesterol. In: Journal of Nutrition and Metabolism. 2010 ; Vol. 2010.
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