Differential expression of gap junction connexins in endocrine and exocrine glands

Paolo Meda, Michael S. Pepper, Otto Traub, Klaus Willecke, Daniel Gros, Eric Beyer, Bruce J Nicholson, David Paul, Lelio Orci

Research output: Contribution to journalArticle

180 Citations (Scopus)

Abstract

We have investigated the expression of three gap junction proteins and their corresponding mRNAs by secretory cells of a variety of endocrine and exocrine rat glands. By immunostaining cryostat sections (indirect immunofluorescence) with antibodies against connexins (Cx) 26, 32, and 43 and by hybridizing total glandular RNA (Northern blot) with cRNAs for these proteins, we have found that several endocrine glands (pituitary, parathyroid, pancreatic islets, and adrenal) express Cx43, variable levels of Cx26, and no Cx32, whereas several exocrine glands (lacrimal gland, salivary glands, pancreas, prostate, and seminal vesicle) express high levels of Cx32 and variable levels of Cx26, but no Cx43. Thus, different sets of proteins comprise the gap junctions of endocrine and exocrine glands. Together with the findings that an endocrine gland (thyroid) that discharges secretory products extracellularly before releasing them in the vascular compartment expresses both Cx43 and Cx32 and that an exocrine gland (preputial gland) that has a pheromonal role expresses Cx43, these observations suggest that the differential expression of gap junction connexins may be required to specify the endocrine or exocrine differentiation of a secretory cell.

Original languageEnglish (US)
Pages (from-to)2371-2378
Number of pages8
JournalEndocrinology
Volume133
Issue number5
StatePublished - Nov 1993
Externally publishedYes

Fingerprint

Exocrine Glands
Endocrine Glands
Connexin 43
Connexins
Gap Junctions
Lacrimal Apparatus
Complementary RNA
Seminal Vesicles
Indirect Fluorescent Antibody Technique
Salivary Glands
Islets of Langerhans
Northern Blotting
Blood Vessels
Prostate
Pancreas
Thyroid Gland
RNA
Messenger RNA
Antibodies
Proteins

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Meda, P., Pepper, M. S., Traub, O., Willecke, K., Gros, D., Beyer, E., ... Orci, L. (1993). Differential expression of gap junction connexins in endocrine and exocrine glands. Endocrinology, 133(5), 2371-2378.

Differential expression of gap junction connexins in endocrine and exocrine glands. / Meda, Paolo; Pepper, Michael S.; Traub, Otto; Willecke, Klaus; Gros, Daniel; Beyer, Eric; Nicholson, Bruce J; Paul, David; Orci, Lelio.

In: Endocrinology, Vol. 133, No. 5, 11.1993, p. 2371-2378.

Research output: Contribution to journalArticle

Meda, P, Pepper, MS, Traub, O, Willecke, K, Gros, D, Beyer, E, Nicholson, BJ, Paul, D & Orci, L 1993, 'Differential expression of gap junction connexins in endocrine and exocrine glands', Endocrinology, vol. 133, no. 5, pp. 2371-2378.
Meda P, Pepper MS, Traub O, Willecke K, Gros D, Beyer E et al. Differential expression of gap junction connexins in endocrine and exocrine glands. Endocrinology. 1993 Nov;133(5):2371-2378.
Meda, Paolo ; Pepper, Michael S. ; Traub, Otto ; Willecke, Klaus ; Gros, Daniel ; Beyer, Eric ; Nicholson, Bruce J ; Paul, David ; Orci, Lelio. / Differential expression of gap junction connexins in endocrine and exocrine glands. In: Endocrinology. 1993 ; Vol. 133, No. 5. pp. 2371-2378.
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