Abstract
We have investigated the expression of three gap junction proteins and their corresponding mRNAs by secretory cells of a variety of endocrine and exocrine rat glands. By immunostaining cryostat sections (indirect immunofluorescence) with antibodies against connexins (Cx) 26, 32, and 43 and by hybridizing total glandular RNA (Northern blot) with cRNAs for these proteins, we have found that several endocrine glands (pituitary, parathyroid, pancreatic islets, and adrenal) express Cx43, variable levels of Cx26, and no Cx32, whereas several exocrine glands (lacrimal gland, salivary glands, pancreas, prostate, and seminal vesicle) express high levels of Cx32 and variable levels of Cx26, but no Cx43. Thus, different sets of proteins comprise the gap junctions of endocrine and exocrine glands. Together with the findings that an endocrine gland (thyroid) that discharges secretory products extracellularly before releasing them in the vascular compartment expresses both Cx43 and Cx32 and that an exocrine gland (preputial gland) that has a pheromonal role expresses Cx43, these observations suggest that the differential expression of gap junction connexins may be required to specify the endocrine or exocrine differentiation of a secretory cell.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2371-2378 |
| Number of pages | 8 |
| Journal | Endocrinology |
| Volume | 133 |
| Issue number | 5 |
| State | Published - Nov 1993 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Endocrinology
- Endocrinology, Diabetes and Metabolism
Cite this
Differential expression of gap junction connexins in endocrine and exocrine glands. / Meda, Paolo; Pepper, Michael S.; Traub, Otto; Willecke, Klaus; Gros, Daniel; Beyer, Eric; Nicholson, Bruce J; Paul, David; Orci, Lelio.
In: Endocrinology, Vol. 133, No. 5, 11.1993, p. 2371-2378.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Differential expression of gap junction connexins in endocrine and exocrine glands
AU - Meda, Paolo
AU - Pepper, Michael S.
AU - Traub, Otto
AU - Willecke, Klaus
AU - Gros, Daniel
AU - Beyer, Eric
AU - Nicholson, Bruce J
AU - Paul, David
AU - Orci, Lelio
PY - 1993/11
Y1 - 1993/11
N2 - We have investigated the expression of three gap junction proteins and their corresponding mRNAs by secretory cells of a variety of endocrine and exocrine rat glands. By immunostaining cryostat sections (indirect immunofluorescence) with antibodies against connexins (Cx) 26, 32, and 43 and by hybridizing total glandular RNA (Northern blot) with cRNAs for these proteins, we have found that several endocrine glands (pituitary, parathyroid, pancreatic islets, and adrenal) express Cx43, variable levels of Cx26, and no Cx32, whereas several exocrine glands (lacrimal gland, salivary glands, pancreas, prostate, and seminal vesicle) express high levels of Cx32 and variable levels of Cx26, but no Cx43. Thus, different sets of proteins comprise the gap junctions of endocrine and exocrine glands. Together with the findings that an endocrine gland (thyroid) that discharges secretory products extracellularly before releasing them in the vascular compartment expresses both Cx43 and Cx32 and that an exocrine gland (preputial gland) that has a pheromonal role expresses Cx43, these observations suggest that the differential expression of gap junction connexins may be required to specify the endocrine or exocrine differentiation of a secretory cell.
AB - We have investigated the expression of three gap junction proteins and their corresponding mRNAs by secretory cells of a variety of endocrine and exocrine rat glands. By immunostaining cryostat sections (indirect immunofluorescence) with antibodies against connexins (Cx) 26, 32, and 43 and by hybridizing total glandular RNA (Northern blot) with cRNAs for these proteins, we have found that several endocrine glands (pituitary, parathyroid, pancreatic islets, and adrenal) express Cx43, variable levels of Cx26, and no Cx32, whereas several exocrine glands (lacrimal gland, salivary glands, pancreas, prostate, and seminal vesicle) express high levels of Cx32 and variable levels of Cx26, but no Cx43. Thus, different sets of proteins comprise the gap junctions of endocrine and exocrine glands. Together with the findings that an endocrine gland (thyroid) that discharges secretory products extracellularly before releasing them in the vascular compartment expresses both Cx43 and Cx32 and that an exocrine gland (preputial gland) that has a pheromonal role expresses Cx43, these observations suggest that the differential expression of gap junction connexins may be required to specify the endocrine or exocrine differentiation of a secretory cell.
UR - http://www.scopus.com/inward/record.url?scp=0027358688&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027358688&partnerID=8YFLogxK
M3 - Article
C2 - 8404689
AN - SCOPUS:0027358688
VL - 133
SP - 2371
EP - 2378
JO - Endocrinology
JF - Endocrinology
SN - 0013-7227
IS - 5
ER -