Differential effects on T cell and NK cell development by tissue-specific expression of H-2D(d) transgene

Maria H. Johansson, Charles Bieberich, Anna Kåse-Sjöström, Takayuki Yoshioka, Elin Höglund, Barbara A. Christy, George Scangos, Klas Kärre, Gilbert Jay, Petter Höglund

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The effect of tissue-specific expression of the MHC class I molecule H-2D(d) on T cell and NK cell specificity was studied in transgenic mice expressing the H-2D(d) gene under the control of the mouse metallothionein-I promoter. MTD mice expressed high amounts of H-2D(d) in the liver, intestine and testis, but only minute amounts in the thymus, spleen and kidney. Zinc administration resulted in a 1.5- and 8.5-fold increase in H-2D(d) expression in the liver and the intestine, respectively, but did not affect expression in the other organs tested. T cell tolerance developed towards H-2D(d) in MTD mice, even in the absence of zinc. In contrast, NK cell-mediated natural resistance against lymphoma grafts was not seen in MTD mice, despite zinc administration. NK cells in MTD mice also failed to develop self tolerance to H-2D(d). The lack of functional effects did not result from inability of NK cells in MTD mice to interact with H-2D(d), as down-regulation of Ly49A receptor expression was observed on liver NK cells in MTD mice. Our data reveal a difference between T cells and NK cells in their requirements for MHC class I molecules in specificity development.

Original languageEnglish (US)
Pages (from-to)525-533
Number of pages9
JournalEuropean Journal of Immunology
Volume30
Issue number2
DOIs
StatePublished - 2000

Keywords

  • MHC class I
  • NK cell
  • T cell
  • Tolerance
  • Transgenic mouse

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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