Differential effects of the pineal gland on LH RH and FSH RH activity in the medial basal hypothalamus of the male golden hamster

D. E. Blask, Russel J Reiter, M. K. Vaughan, Linda Y Johnson

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Abstract

Crude neutralized acid extracts were made of medial basal hypothalamic (MBH) tissue obtained from adult male hamsters which had been either blinded, blinded and pinealectomized or left intact. After 11 weeks of tretment, the reproductive organs of the blind animals were markedly involuted compared to intact controls, an effect completely reversed by pinealectomy. The luteinizing hormone-releasing hormone (LH-RH) and follicle-stimulating hormone-releasing hormone (FSH-RH) activity of the extracts was assessed using an in vitro system in which LH and FSH release into the medium from hemipituitaries, derived from ovariectomized, estrogen-progesterone-treated female rats, was measured by radioimmunoassay (RIA), LH and FSH release was responsive to synthetic LH-RH in a dose-related manner; however, in the latter case there were no statistically significant differences between the FSH responses to the various doses of LH-RH. The LH-RH activity of hypothalamic extracts from blind hamsters was depressed as compared with intact controls; however, this effect was not reversed by pinealectomy. The FSH-RH activity of hypothalamic extract from blind hamsters was virtually eliminated compared with that in hypothalami of intact controls. Unlike with LH-RH activity, pinealectomy completely reversed the effects of light deprivation on FSH-RH activity with levels returning to normal. These results indicate that the pineal gland of the male hamster is capable of influencing the hypothalamic levels of FSH-RH activity independent of LH-RH activity.

Original languageEnglish (US)
Pages (from-to)36-43
Number of pages8
JournalNeuroendocrinology
Volume28
Issue number1
StatePublished - 1979

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Middle Hypothalamus
Pineal Gland
Mesocricetus
Gonadotropin-Releasing Hormone
Follicle Stimulating Hormone
Cricetinae
Hormones
Pituitary Hormone-Releasing Hormones
Animal Structures
Hypothalamus
Radioimmunoassay
Progesterone
Estrogens
Light
Acids

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)

Cite this

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title = "Differential effects of the pineal gland on LH RH and FSH RH activity in the medial basal hypothalamus of the male golden hamster",
abstract = "Crude neutralized acid extracts were made of medial basal hypothalamic (MBH) tissue obtained from adult male hamsters which had been either blinded, blinded and pinealectomized or left intact. After 11 weeks of tretment, the reproductive organs of the blind animals were markedly involuted compared to intact controls, an effect completely reversed by pinealectomy. The luteinizing hormone-releasing hormone (LH-RH) and follicle-stimulating hormone-releasing hormone (FSH-RH) activity of the extracts was assessed using an in vitro system in which LH and FSH release into the medium from hemipituitaries, derived from ovariectomized, estrogen-progesterone-treated female rats, was measured by radioimmunoassay (RIA), LH and FSH release was responsive to synthetic LH-RH in a dose-related manner; however, in the latter case there were no statistically significant differences between the FSH responses to the various doses of LH-RH. The LH-RH activity of hypothalamic extracts from blind hamsters was depressed as compared with intact controls; however, this effect was not reversed by pinealectomy. The FSH-RH activity of hypothalamic extract from blind hamsters was virtually eliminated compared with that in hypothalami of intact controls. Unlike with LH-RH activity, pinealectomy completely reversed the effects of light deprivation on FSH-RH activity with levels returning to normal. These results indicate that the pineal gland of the male hamster is capable of influencing the hypothalamic levels of FSH-RH activity independent of LH-RH activity.",
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T1 - Differential effects of the pineal gland on LH RH and FSH RH activity in the medial basal hypothalamus of the male golden hamster

AU - Blask, D. E.

AU - Reiter, Russel J

AU - Vaughan, M. K.

AU - Johnson, Linda Y

PY - 1979

Y1 - 1979

N2 - Crude neutralized acid extracts were made of medial basal hypothalamic (MBH) tissue obtained from adult male hamsters which had been either blinded, blinded and pinealectomized or left intact. After 11 weeks of tretment, the reproductive organs of the blind animals were markedly involuted compared to intact controls, an effect completely reversed by pinealectomy. The luteinizing hormone-releasing hormone (LH-RH) and follicle-stimulating hormone-releasing hormone (FSH-RH) activity of the extracts was assessed using an in vitro system in which LH and FSH release into the medium from hemipituitaries, derived from ovariectomized, estrogen-progesterone-treated female rats, was measured by radioimmunoassay (RIA), LH and FSH release was responsive to synthetic LH-RH in a dose-related manner; however, in the latter case there were no statistically significant differences between the FSH responses to the various doses of LH-RH. The LH-RH activity of hypothalamic extracts from blind hamsters was depressed as compared with intact controls; however, this effect was not reversed by pinealectomy. The FSH-RH activity of hypothalamic extract from blind hamsters was virtually eliminated compared with that in hypothalami of intact controls. Unlike with LH-RH activity, pinealectomy completely reversed the effects of light deprivation on FSH-RH activity with levels returning to normal. These results indicate that the pineal gland of the male hamster is capable of influencing the hypothalamic levels of FSH-RH activity independent of LH-RH activity.

AB - Crude neutralized acid extracts were made of medial basal hypothalamic (MBH) tissue obtained from adult male hamsters which had been either blinded, blinded and pinealectomized or left intact. After 11 weeks of tretment, the reproductive organs of the blind animals were markedly involuted compared to intact controls, an effect completely reversed by pinealectomy. The luteinizing hormone-releasing hormone (LH-RH) and follicle-stimulating hormone-releasing hormone (FSH-RH) activity of the extracts was assessed using an in vitro system in which LH and FSH release into the medium from hemipituitaries, derived from ovariectomized, estrogen-progesterone-treated female rats, was measured by radioimmunoassay (RIA), LH and FSH release was responsive to synthetic LH-RH in a dose-related manner; however, in the latter case there were no statistically significant differences between the FSH responses to the various doses of LH-RH. The LH-RH activity of hypothalamic extracts from blind hamsters was depressed as compared with intact controls; however, this effect was not reversed by pinealectomy. The FSH-RH activity of hypothalamic extract from blind hamsters was virtually eliminated compared with that in hypothalami of intact controls. Unlike with LH-RH activity, pinealectomy completely reversed the effects of light deprivation on FSH-RH activity with levels returning to normal. These results indicate that the pineal gland of the male hamster is capable of influencing the hypothalamic levels of FSH-RH activity independent of LH-RH activity.

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