Differential effects of protein kinase C on the levels of epithelial Na+ channel subunit proteins

J. D. Stockand, B. Hui-Fang, J. Schenck, B. Malik, P. Middleton, L. E. Schlanger, D. C. Eaton

Research output: Contribution to journalArticle

76 Scopus citations

Abstract

Regulation of epithelial Na+ channel (ENaC) subunit levels by protein kinase C (PKC) was investigated in A6 cells. PKC activation altered ENaC subunit levels, differentially decreasing the levels of both β and γ, but not αENaC. Temporal regulation of β and γENaC by PKC differed; γENaC decreased with a time constant of3.7 ± 1.0 h, whereas βENaC decreased in 13.9 ±3.0h. Activation of PKC also resulted in a decrease in trans-epithelial Na+ reabsorption for up to 48h. PMA activation of PKC resulted in negative feedback inhibition of PKC protein levels beginning within 4h. Both β and γENaC levels, as well as transport tended toward pretreatment values after 48 h of PMA treatment. PKC inhibitors attenuated the effects of PMA on ENaC subunit levels and Na+ transport. These results directly show for the first time that PKC differentially regulates ENaC subunit levels by decreasing the levels of β and γ but not αENaC protein. These results imply a PKC-dependent, long term decrease in Na+ reabsorption.

Original languageEnglish (US)
Pages (from-to)25760-25765
Number of pages6
JournalJournal of Biological Chemistry
Volume275
Issue number33
DOIs
StatePublished - Aug 18 2000

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Stockand, J. D., Hui-Fang, B., Schenck, J., Malik, B., Middleton, P., Schlanger, L. E., & Eaton, D. C. (2000). Differential effects of protein kinase C on the levels of epithelial Na+ channel subunit proteins. Journal of Biological Chemistry, 275(33), 25760-25765. https://doi.org/10.1074/jbc.M003615200