Differential Binding of the Fluorescent Probe 8-Anilinonaphthalene-2-sulfonic Acid to Rhodanese Catalytic Intermediates

Paul M. Horowitz, Nick L. Criscimagna

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Studies have been performed to quantitate the binding of the fluorescent probe 8-anilinonaphthalene-2-sulfonic acid (2,8-ANS) to catalytic intermediates of the enzyme rhodanese: the sulfur-substituted form (ES) and the sulfur-free form (E). The molecule 2,8-ANS has not been extensively used for protein studies, and some characterization is presented to demonstrate its usefulness as a probe for apolar binding sites. The molecule 2,8-ANS binds to at least two classes of sites on rhodanese. One class (class 1) is present in the ES form and has a Kd of 1.7 mM. The E form of rhodanese appears to have a second class of sites (class 2) in addition to the class 1 sites. Two independent fluorometric methods of analyzing the class 2 binding of 2,8-ANS to the E form gave an average value for Kd ≃179 μM. These fluorometric titrations, together with a Job plot, clearly indicate that 2,8-ANS binds to more than one site on the E form of rhodanese. The apparent apolarity is slightly higher for class 2 sites than for the class 1 sites, but both give Z factors of >85. The substrate thiosulfate is able to displace the probe that is bound to the class 2 sites on the E form of the enzyme. Further, 2,8-ANS is found to be a competitive inhibitor of the catalyzed reaction with an apparent Kd of 170 μM. Circular dichroism measurements detect no significant changes in the average conformation of rhodanese that can be ascribed to the presence of 2,8-ANS. Ultracentrifugation studies show that there is no aggregation of the normally monomeric rhodanese attributable to 2,8-ANS binding. The results are interpreted in terms of a model in which the two-domain structure, into which rhodanese is folded, participates in a catalytically important conformational change that is associated with alterations in solute-accessible apparent apolarity.

Original languageEnglish (US)
Pages (from-to)2587-2593
Number of pages7
Issue number11
StatePublished - May 1 1985
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry


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