Differential bile-pancreatic secretory effects of CCK-58 and CCK-8

Joseph R. Reeve; S. Vincent Wu; David A. Keire; Kym Faull; Peter Chew; Travis E. Solomon; Gary M. Green; Tamer Coskun

doi:10.1152/ajpgi.00020.2003

Differential bile-pancreatic secretory effects of CCK-58 and CCK-8

Joseph R. Reeve, S. Vincent Wu, David A. Keire, Kym Faull, Peter Chew, Travis E. Solomon, Gary M. Green, Tamer Coskun

Department of Cellular & Integrative Physiology

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

In this work, we 1) synthesized rat CCK-58, 2) determined the amounts and forms of rat CCK in whole blood after stimulation of its release by casein, 3) determined the potency of CCK-8 and CCK-58 peptides to displace labeled CCK-8 from CCK_A and CCK_B receptors transfected into Chinese hamster ovary (CHO) cells, and 4) examined the biological actions of CCK-8 and rat CCK-58 in an anesthetized rat model. CCK-58 was the only detected endocrine form of CCK in rat blood. Synthetic rat CCK-58 was less potent than CCK-8 for displacing the label from CCK_A and CCK_B receptors in transfected CHO cells. However, rat CCK-58 was more potent than CCK-8 for stimulation of pancreatic protein secretion in the anesthetized rat. In addition, CCK-58 but not CCK-8 stimulated fluid secretion in this anesthetized rat model. These data suggest that regions outside the COOH terminus of rat CCK-58 influence the expression of CCK biological activity. The presence of only CCK-58 in the circulation and the fact that its biological activity differs from CCK-8 suggests that CCK-58 deserves scrutiny in other physiological models of CCK activity.

Original language	English (US)
Pages (from-to)	G395-G402
Journal	American Journal of Physiology - Gastrointestinal and Liver Physiology
Volume	286
Issue number	3 49-3
DOIs	https://doi.org/10.1152/ajpgi.00020.2003
State	Published - Mar 2004

Keywords

Cholecystokinin

ASJC Scopus subject areas

Physiology
Hepatology
Gastroenterology
Physiology (medical)

Access to Document

10.1152/ajpgi.00020.2003

Cite this

@article{dba9ec5a5bb6408388e5040a0713ed18,

title = "Differential bile-pancreatic secretory effects of CCK-58 and CCK-8",

abstract = "In this work, we 1) synthesized rat CCK-58, 2) determined the amounts and forms of rat CCK in whole blood after stimulation of its release by casein, 3) determined the potency of CCK-8 and CCK-58 peptides to displace labeled CCK-8 from CCKA and CCKB receptors transfected into Chinese hamster ovary (CHO) cells, and 4) examined the biological actions of CCK-8 and rat CCK-58 in an anesthetized rat model. CCK-58 was the only detected endocrine form of CCK in rat blood. Synthetic rat CCK-58 was less potent than CCK-8 for displacing the label from CCKA and CCKB receptors in transfected CHO cells. However, rat CCK-58 was more potent than CCK-8 for stimulation of pancreatic protein secretion in the anesthetized rat. In addition, CCK-58 but not CCK-8 stimulated fluid secretion in this anesthetized rat model. These data suggest that regions outside the COOH terminus of rat CCK-58 influence the expression of CCK biological activity. The presence of only CCK-58 in the circulation and the fact that its biological activity differs from CCK-8 suggests that CCK-58 deserves scrutiny in other physiological models of CCK activity.",

keywords = "Cholecystokinin",

author = "Reeve, {Joseph R.} and Wu, {S. Vincent} and Keire, {David A.} and Kym Faull and Peter Chew and Solomon, {Travis E.} and Green, {Gary M.} and Tamer Coskun",

year = "2004",

month = mar,

doi = "10.1152/ajpgi.00020.2003",

language = "English (US)",

volume = "286",

pages = "G395--G402",

journal = "American Journal of Physiology - Renal Physiology",

issn = "0363-6127",

publisher = "American Physiological Society",

number = "3 49-3",

}

TY - JOUR

T1 - Differential bile-pancreatic secretory effects of CCK-58 and CCK-8

AU - Reeve, Joseph R.

AU - Wu, S. Vincent

AU - Keire, David A.

AU - Faull, Kym

AU - Chew, Peter

AU - Solomon, Travis E.

AU - Green, Gary M.

AU - Coskun, Tamer

PY - 2004/3

Y1 - 2004/3

N2 - In this work, we 1) synthesized rat CCK-58, 2) determined the amounts and forms of rat CCK in whole blood after stimulation of its release by casein, 3) determined the potency of CCK-8 and CCK-58 peptides to displace labeled CCK-8 from CCKA and CCKB receptors transfected into Chinese hamster ovary (CHO) cells, and 4) examined the biological actions of CCK-8 and rat CCK-58 in an anesthetized rat model. CCK-58 was the only detected endocrine form of CCK in rat blood. Synthetic rat CCK-58 was less potent than CCK-8 for displacing the label from CCKA and CCKB receptors in transfected CHO cells. However, rat CCK-58 was more potent than CCK-8 for stimulation of pancreatic protein secretion in the anesthetized rat. In addition, CCK-58 but not CCK-8 stimulated fluid secretion in this anesthetized rat model. These data suggest that regions outside the COOH terminus of rat CCK-58 influence the expression of CCK biological activity. The presence of only CCK-58 in the circulation and the fact that its biological activity differs from CCK-8 suggests that CCK-58 deserves scrutiny in other physiological models of CCK activity.

AB - In this work, we 1) synthesized rat CCK-58, 2) determined the amounts and forms of rat CCK in whole blood after stimulation of its release by casein, 3) determined the potency of CCK-8 and CCK-58 peptides to displace labeled CCK-8 from CCKA and CCKB receptors transfected into Chinese hamster ovary (CHO) cells, and 4) examined the biological actions of CCK-8 and rat CCK-58 in an anesthetized rat model. CCK-58 was the only detected endocrine form of CCK in rat blood. Synthetic rat CCK-58 was less potent than CCK-8 for displacing the label from CCKA and CCKB receptors in transfected CHO cells. However, rat CCK-58 was more potent than CCK-8 for stimulation of pancreatic protein secretion in the anesthetized rat. In addition, CCK-58 but not CCK-8 stimulated fluid secretion in this anesthetized rat model. These data suggest that regions outside the COOH terminus of rat CCK-58 influence the expression of CCK biological activity. The presence of only CCK-58 in the circulation and the fact that its biological activity differs from CCK-8 suggests that CCK-58 deserves scrutiny in other physiological models of CCK activity.

KW - Cholecystokinin

UR - http://www.scopus.com/inward/record.url?scp=1342322890&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1342322890&partnerID=8YFLogxK

U2 - 10.1152/ajpgi.00020.2003

DO - 10.1152/ajpgi.00020.2003

M3 - Article

C2 - 14604858

AN - SCOPUS:1342322890

VL - 286

SP - G395-G402

JO - American Journal of Physiology - Renal Physiology

JF - American Journal of Physiology - Renal Physiology

SN - 0363-6127

IS - 3 49-3

ER -

Differential bile-pancreatic secretory effects of CCK-58 and CCK-8

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this