Differential bile-pancreatic secretory effects of CCK-58 and CCK-8

Joseph R. Reeve, S. Vincent Wu, David A. Keire, Kym Faull, Peter Chew, Travis E. Solomon, Gary M. Green, Tamer Coskun

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

In this work, we 1) synthesized rat CCK-58, 2) determined the amounts and forms of rat CCK in whole blood after stimulation of its release by casein, 3) determined the potency of CCK-8 and CCK-58 peptides to displace labeled CCK-8 from CCKA and CCKB receptors transfected into Chinese hamster ovary (CHO) cells, and 4) examined the biological actions of CCK-8 and rat CCK-58 in an anesthetized rat model. CCK-58 was the only detected endocrine form of CCK in rat blood. Synthetic rat CCK-58 was less potent than CCK-8 for displacing the label from CCKA and CCKB receptors in transfected CHO cells. However, rat CCK-58 was more potent than CCK-8 for stimulation of pancreatic protein secretion in the anesthetized rat. In addition, CCK-58 but not CCK-8 stimulated fluid secretion in this anesthetized rat model. These data suggest that regions outside the COOH terminus of rat CCK-58 influence the expression of CCK biological activity. The presence of only CCK-58 in the circulation and the fact that its biological activity differs from CCK-8 suggests that CCK-58 deserves scrutiny in other physiological models of CCK activity.

Original languageEnglish (US)
Pages (from-to)G395-G402
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume286
Issue number3 49-3
DOIs
StatePublished - Mar 2004

Keywords

  • Cholecystokinin

ASJC Scopus subject areas

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)

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