Differential appearance of DNase I-hypersensitive sites correlates with differential transcription of Pgk genes during spermatogenesis in the mouse

Meena Kumari, James C. Stroud, Antje Anji, John R. McCarrey

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Two functional genes encoding phosphoglycerate kinase are differentially expressed during spermatogenesis in the mouse. Expression of the X-linked Pgk-1 gene is repressed coincident with X chromosome inactivation during prophase of meiosis I. At this same stage, expression of the autosomal Pgk-2 gene is initiated by tissue-specific mechanisms. To investigate the role of chromatin structure in these processes, we have examined the appearance and disappearance of DNase I-hypersensitive (DH) sites in each gene, and correlated this with transcriptional activity as measured by nuclear run-off analysis at specific stages of spermatogenesis. Our results demonstrate that the occurrence of DH sites is related to periods of active transcription. Results with the Pgk-1 gene indicate that transcriptional inactivation of the X chromosome in spermatogenic cells may not he as complete as that in somatic cells, and that maximum repression may be limited to a very transient period during the pachytene stage of first meiotic prophase. Results with the Pgk-2 gene indicate that DH sites appear coincident with, or just prior to, transcriptional activation of this gene. The implications of these results are discussed with respect to the role of X chromosome inactivation in spermatogenic cells and the developmental order of molecular events that regulate differential gene expression during spermatogenesis.

Original languageEnglish (US)
Pages (from-to)14390-14397
Number of pages8
JournalJournal of Biological Chemistry
Volume271
Issue number24
DOIs
StatePublished - 1996
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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