Different ratios of eicosapentaenoic and docosahexaenoic omega-3 fatty acids in commercial fish oils differentially alter pro-inflammatory cytokines in peritoneal macrophages from C57BL/6 female mice

Arunabh Bhattacharya, Dongxu Sun, Mizanur Rahman, Gabriel Fernandes

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

The use of fish oil (FO) as a dietary supplement to prevent or reduce the severity of cardiovascular diseases and autoimmune disorders such as rheumatoid arthritis is receiving much attention. Several recent reports indicate that eating fish often or the use of small doses of FO capsules appears to have benefits against cardiovascular diseases. We have reported in the past that diets enriched with FO protect against renal diseases and prolong the life span of autoimmune-prone mice compared to corn oil (CO) diets. However, the optimum ratio of eicosapentaenoic acid (EPA) to docosahexaenoic acid (DHA) in commercially available FOs to reduce the production of various pro-inflammatory cytokines has not been well established. We, therefore, obtained deodorized FO from three sources containing different EPA/DHA contents, fed them to C57BL/6 mice for 8 weeks in a 10% (vol/wt) diet (oil A, 11/10; oil B, 14/9; oil C, 23/14) and compared them with (10%) CO-fed mice as control. TNF-α, IL-6 and IL-1β were measured by enzyme-linked immunosorbent assay in thioglycollate-induced macrophages, 8 and 24 h after lipopolysaccharide treatment. The results showed a significant decrease in TNF-α after only 8 h in oil C. After 24 h, TNF-α, IL-6 and IL-1β levels decreased only in mice fed oil C, although nonsignificant decreases were seen in mice fed oil A compared to mice fed CO. The antioxidant enzymes, catalase and glutathione transferase, were higher in kidneys of mice fed oil C compared to mice fed CO. The study suggests that anti-inflammatory activity may vary among different sources of FO due to variations in EPA/DHA content.

Original languageEnglish (US)
Pages (from-to)23-30
Number of pages8
JournalJournal of Nutritional Biochemistry
Volume18
Issue number1
DOIs
StatePublished - Jan 2007

Fingerprint

Industrial Oils
Fish Oils
Macrophages
Omega-3 Fatty Acids
Peritoneal Macrophages
Corn Oil
Oils
Eicosapentaenoic Acid
Docosahexaenoic Acids
Cytokines
Nutrition
Interleukin-1
Interleukin-6
Dietary supplements
Diet
Thioglycolates
Immunosorbents
Cardiovascular Diseases
Enzymes
Glutathione Transferase

Keywords

  • Antioxidant enzymes
  • Cytokines
  • Fatty acids
  • Fish oil
  • Peritoneal macrophages

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Different ratios of eicosapentaenoic and docosahexaenoic omega-3 fatty acids in commercial fish oils differentially alter pro-inflammatory cytokines in peritoneal macrophages from C57BL/6 female mice. / Bhattacharya, Arunabh; Sun, Dongxu; Rahman, Mizanur; Fernandes, Gabriel.

In: Journal of Nutritional Biochemistry, Vol. 18, No. 1, 01.2007, p. 23-30.

Research output: Contribution to journalArticle

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abstract = "The use of fish oil (FO) as a dietary supplement to prevent or reduce the severity of cardiovascular diseases and autoimmune disorders such as rheumatoid arthritis is receiving much attention. Several recent reports indicate that eating fish often or the use of small doses of FO capsules appears to have benefits against cardiovascular diseases. We have reported in the past that diets enriched with FO protect against renal diseases and prolong the life span of autoimmune-prone mice compared to corn oil (CO) diets. However, the optimum ratio of eicosapentaenoic acid (EPA) to docosahexaenoic acid (DHA) in commercially available FOs to reduce the production of various pro-inflammatory cytokines has not been well established. We, therefore, obtained deodorized FO from three sources containing different EPA/DHA contents, fed them to C57BL/6 mice for 8 weeks in a 10{\%} (vol/wt) diet (oil A, 11/10; oil B, 14/9; oil C, 23/14) and compared them with (10{\%}) CO-fed mice as control. TNF-α, IL-6 and IL-1β were measured by enzyme-linked immunosorbent assay in thioglycollate-induced macrophages, 8 and 24 h after lipopolysaccharide treatment. The results showed a significant decrease in TNF-α after only 8 h in oil C. After 24 h, TNF-α, IL-6 and IL-1β levels decreased only in mice fed oil C, although nonsignificant decreases were seen in mice fed oil A compared to mice fed CO. The antioxidant enzymes, catalase and glutathione transferase, were higher in kidneys of mice fed oil C compared to mice fed CO. The study suggests that anti-inflammatory activity may vary among different sources of FO due to variations in EPA/DHA content.",
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