Dietary fat and cholesterol effects on plasma low density lipoproteins in pedigreed baboons

Amareshwar T K Singh, David L. Rainwater, Candace M. Kummerer, R. Mark Sharp, Mahmnod Poushesh, Wendy R. Shelledy, John L. Vandeberg

Research output: Contribution to journalArticle

Abstract

To determine dietary and genetic effects on LDL phenotypes, such as LDL-C and apoB concentrations, particle diameters and LDL-C size distribution, 150 pedigreed baboons, members of 19 sire groups, were fed a sequence of three diets differing in fat and cholesterol levels. LDL-C was measured by the use of heparin-Mn2+, apoB by competitive immunoassay, and LDL particle diameters were characterized by nondenaturing gradient gel electrophoresis and densitometry. Elevating dietary fat from 10% to 40% of calories, resulted in modest, but significant, increases of all LDL phenotype measures; whereas, elevating the level of cholesterol in the high fat diet resulted in relatively larger increases. There were significant sire effects and sire x diet interactions for the LDL measures. The results of the present study indicate genes affect the expression of LDL phenotypes on each of the three diets, as well as the patterns of response to dietary changes.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - 1996
Externally publishedYes

Fingerprint

Dietary Cholesterol
Papio
Dietary Fats
low density lipoprotein
LDL Lipoproteins
dietary fat
sires
cholesterol
Plasmas
phenotype
Nutrition
densitometry
heparin
high fat diet
immunoassays
eating habits
diet
gel electrophoresis
Apolipoproteins B
Diet

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Singh, A. T. K., Rainwater, D. L., Kummerer, C. M., Mark Sharp, R., Poushesh, M., Shelledy, W. R., & Vandeberg, J. L. (1996). Dietary fat and cholesterol effects on plasma low density lipoproteins in pedigreed baboons. FASEB Journal, 10(3).

Dietary fat and cholesterol effects on plasma low density lipoproteins in pedigreed baboons. / Singh, Amareshwar T K; Rainwater, David L.; Kummerer, Candace M.; Mark Sharp, R.; Poushesh, Mahmnod; Shelledy, Wendy R.; Vandeberg, John L.

In: FASEB Journal, Vol. 10, No. 3, 1996.

Research output: Contribution to journalArticle

Singh, ATK, Rainwater, DL, Kummerer, CM, Mark Sharp, R, Poushesh, M, Shelledy, WR & Vandeberg, JL 1996, 'Dietary fat and cholesterol effects on plasma low density lipoproteins in pedigreed baboons', FASEB Journal, vol. 10, no. 3.
Singh ATK, Rainwater DL, Kummerer CM, Mark Sharp R, Poushesh M, Shelledy WR et al. Dietary fat and cholesterol effects on plasma low density lipoproteins in pedigreed baboons. FASEB Journal. 1996;10(3).
Singh, Amareshwar T K ; Rainwater, David L. ; Kummerer, Candace M. ; Mark Sharp, R. ; Poushesh, Mahmnod ; Shelledy, Wendy R. ; Vandeberg, John L. / Dietary fat and cholesterol effects on plasma low density lipoproteins in pedigreed baboons. In: FASEB Journal. 1996 ; Vol. 10, No. 3.
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