Dietary calorie restriction does not affect the levels of protein elongation factors in rat livers during ageing

Suresh I.S. Rattan, Walter F. Ward, Marianne Glenting, Lise Svendsen, Bent Riis, Brian F.C. Clark

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Dietary calorie restriction of rats has been previously shown to increase protein synthetic rates in liver and kidney cells during ageing. Here we have compared the activiy and amounts of active elongation factors EF-1α and EF-2 in cell-free extracts prepared from livers isolated from male Fischer 344 rats of different ages. Although there is some age-related decline in the catalytic activity and amounts of active EF-1α during ageing, no differences between freely-fed and calorie-restricted animals were observed. In the case of EF-2, the amounts of ADP-ribosylatable EF-2 neither declined during ageing nor differed between freely-fed and calorie-restricted animals. Thus differences in the protein synthetic rates in calorie-restricted and freely-fed rats are not reflected at the level of protein elongation factors, and may involve some other mechanisms of regulation.

Original languageEnglish (US)
Pages (from-to)85-91
Number of pages7
JournalMechanisms of Ageing and Development
Volume58
Issue number1
DOIs
StatePublished - Apr 1 1991

Keywords

  • Ageing
  • Calorie restriction
  • Elongation factors
  • Protein synthesis

ASJC Scopus subject areas

  • Aging
  • Developmental Biology

Fingerprint Dive into the research topics of 'Dietary calorie restriction does not affect the levels of protein elongation factors in rat livers during ageing'. Together they form a unique fingerprint.

  • Cite this