Dibutyryl‐Cyclic GMP Stimulation of Ca2+‐ATPase Activity in Rat Brain Synaptic Membranes

Kenneth A. Stauderman, David J. Jones, David H. Ross

Research output: Contribution to journalArticle

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Abstract

Abstract: The effects of dibutyryl cyclic AMP (dbcAMP) and dibutyryl cyclic GMP (db‐cGMP) were tested on Ca2+‐ATPase, Mg2+‐ATPase, and (Ca2++ Mg2+)‐ATPase activities in lysed synaptosomes prepared from whole rat brains (minus cerebellum). At concentrations from 0.1 to 2.0 mM, db‐cGMP produced a selective, concentration‐dependent increase in Ca2+‐ATPase activity. Both db‐cGMP and db‐cAMP slightly reduced Mg2+‐ATPase activity, whereas neither compound had concentration‐dependent effects on (Ca2++ Mg2+)‐ATPase activity. These findings suggest that the Mg2+‐independent, Ca2+‐ATPase activity in rat brain is regulated by a cyclic GMP‐dependent process. Further, the data provide evidence that the Ca2+‐ATPase activity in lysed synaptosomal membranes represents an enzyme that is distinguishable from both the Mg2+‐ and (Ca2++ Mg2+‐ATPase.

Original languageEnglish (US)
Pages (from-to)970-972
Number of pages3
JournalJournal of Neurochemistry
Volume45
Issue number3
DOIs
StatePublished - Sep 1985

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Keywords

  • (Ca+ Mg)‐ATPase
  • Ca‐ATPase
  • Mg‐ATPase
  • db‐cAMP–db‐cGMP

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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