Abstract
Abstract: The effects of dibutyryl cyclic AMP (dbcAMP) and dibutyryl cyclic GMP (db‐cGMP) were tested on Ca2+‐ATPase, Mg2+‐ATPase, and (Ca2++ Mg2+)‐ATPase activities in lysed synaptosomes prepared from whole rat brains (minus cerebellum). At concentrations from 0.1 to 2.0 mM, db‐cGMP produced a selective, concentration‐dependent increase in Ca2+‐ATPase activity. Both db‐cGMP and db‐cAMP slightly reduced Mg2+‐ATPase activity, whereas neither compound had concentration‐dependent effects on (Ca2++ Mg2+)‐ATPase activity. These findings suggest that the Mg2+‐independent, Ca2+‐ATPase activity in rat brain is regulated by a cyclic GMP‐dependent process. Further, the data provide evidence that the Ca2+‐ATPase activity in lysed synaptosomal membranes represents an enzyme that is distinguishable from both the Mg2+‐ and (Ca2++ Mg2+‐ATPase.
Original language | English (US) |
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Pages (from-to) | 970-972 |
Number of pages | 3 |
Journal | Journal of Neurochemistry |
Volume | 45 |
Issue number | 3 |
DOIs | |
State | Published - Sep 1985 |
Keywords
- (Ca+ Mg)‐ATPase
- Ca‐ATPase
- Mg‐ATPase
- db‐cAMP–db‐cGMP
ASJC Scopus subject areas
- Biochemistry
- Cellular and Molecular Neuroscience