Abstract: The effects of dibutyryl cyclic AMP (dbcAMP) and dibutyryl cyclic GMP (db‐cGMP) were tested on Ca2+‐ATPase, Mg2+‐ATPase, and (Ca2++ Mg2+)‐ATPase activities in lysed synaptosomes prepared from whole rat brains (minus cerebellum). At concentrations from 0.1 to 2.0 mM, db‐cGMP produced a selective, concentration‐dependent increase in Ca2+‐ATPase activity. Both db‐cGMP and db‐cAMP slightly reduced Mg2+‐ATPase activity, whereas neither compound had concentration‐dependent effects on (Ca2++ Mg2+)‐ATPase activity. These findings suggest that the Mg2+‐independent, Ca2+‐ATPase activity in rat brain is regulated by a cyclic GMP‐dependent process. Further, the data provide evidence that the Ca2+‐ATPase activity in lysed synaptosomal membranes represents an enzyme that is distinguishable from both the Mg2+‐ and (Ca2++ Mg2+‐ATPase.
|Original language||English (US)|
|Number of pages||3|
|Journal||Journal of Neurochemistry|
|State||Published - Sep 1985|
- (Ca+ Mg)‐ATPase
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience