Diaphragmatic function after resistive breathing in vitamin E-deficient rats

Antonio R Anzueto, F. H. Andrade, L. C. Maxwell, Stephanie M Levine, R. A. Lawrence, S. G. Jenkinson

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The effects of vitamin E deficiency on diaphragm function were studied at rest and after resistive breathing (RB) in Sprague-Dawley rats (wt 300-400 g). The animals were pair fed a vitamin E-deficient diet (E-def) or a matched vitamin E-sufficient diet (E-suf). Each diet group was then further subdivided into a group that breathed unimpeded (control) and a second group that breathed through an inspiratory resistor until the animals were unable to sustain 70% of their maximum airway pressure. Diaphragm samples were obtained for analysis of thiobarbituric acid-reactive substances, glutathione (GSH) concentrations, and glutathione disulfide (GSSG) concentrations. In vitro isometric contractile studies were also performed and included twitch (P(t)) and maximum tetanic (P(o)) tensions, force-frequency curves, fatigue index, and recovery index. P(t) was significantly reduced in the E-suf RB group as well as both of the E-def groups. P(o) was also significantly reduced in both E-def groups. The E-def rats subjected to RB showed a significant decrease in tension at both high and low frequencies compared with the E-suf rats. Concentrations of diaphragm thiobarbituric acid-reactive substances were significantly increased in both E-def groups. RB in both E- suf and E-def rats resulted in increases in diaphragm concentrations of GSSG and decreases in the GSH/GSSG ratios. We conclude that reduction of contractile function, lipid peroxidation, and activation of the GSH redox cycle occur with RB and that these effects are significantly increased in the presence of vitamin E deficiency.

Original languageEnglish (US)
Pages (from-to)267-271
Number of pages5
JournalJournal of Applied Physiology
Volume74
Issue number1
StatePublished - 1993

Fingerprint

Vitamin E
Glutathione Disulfide
Respiration
Diaphragm
Vitamin E Deficiency
Thiobarbituric Acid Reactive Substances
Diet
Lipid Peroxidation
Oxidation-Reduction
Fatigue
Glutathione
Sprague Dawley Rats
Pressure

Keywords

  • contractile properties
  • diaphragm
  • fatigue
  • free radicals
  • glutathione
  • lipid peroxidation
  • respiratory muscle
  • vitamin E deficiency

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Anzueto, A. R., Andrade, F. H., Maxwell, L. C., Levine, S. M., Lawrence, R. A., & Jenkinson, S. G. (1993). Diaphragmatic function after resistive breathing in vitamin E-deficient rats. Journal of Applied Physiology, 74(1), 267-271.

Diaphragmatic function after resistive breathing in vitamin E-deficient rats. / Anzueto, Antonio R; Andrade, F. H.; Maxwell, L. C.; Levine, Stephanie M; Lawrence, R. A.; Jenkinson, S. G.

In: Journal of Applied Physiology, Vol. 74, No. 1, 1993, p. 267-271.

Research output: Contribution to journalArticle

Anzueto, AR, Andrade, FH, Maxwell, LC, Levine, SM, Lawrence, RA & Jenkinson, SG 1993, 'Diaphragmatic function after resistive breathing in vitamin E-deficient rats', Journal of Applied Physiology, vol. 74, no. 1, pp. 267-271.
Anzueto, Antonio R ; Andrade, F. H. ; Maxwell, L. C. ; Levine, Stephanie M ; Lawrence, R. A. ; Jenkinson, S. G. / Diaphragmatic function after resistive breathing in vitamin E-deficient rats. In: Journal of Applied Physiology. 1993 ; Vol. 74, No. 1. pp. 267-271.
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