Diagnostic evaluation of the amastin protein from Leishmania infantum in canine and human visceral leishmaniasis and immunogenicity in human cells derived from patients and healthy controls

Danniele L. Vale; Daniel S. Dias; Amanda S. Machado; Patrícia A.F. Ribeiro; Daniela P. Lage; Lourena E. Costa; Bethina T. Steiner; Grasiele S.V. Tavares; Fernanda F. Ramos; Abel Martínez-Rodrigo; Miguel A. Chávez-Fumagalli; Rachel B. Caligiorne; Danielle F. de Magalhães-Soares; Julia A.G. Silveira; Ricardo A. Machado-de-Ávila; Antônio L. Teixeira; Eduardo A.F. Coelho

doi:10.1016/j.diagmicrobio.2019.04.015

Diagnostic evaluation of the amastin protein from Leishmania infantum in canine and human visceral leishmaniasis and immunogenicity in human cells derived from patients and healthy controls

Danniele L. Vale, Daniel S. Dias, Amanda S. Machado, Patrícia A.F. Ribeiro, Daniela P. Lage, Lourena E. Costa, Bethina T. Steiner, Grasiele S.V. Tavares, Fernanda F. Ramos, Abel Martínez-Rodrigo, Miguel A. Chávez-Fumagalli, Rachel B. Caligiorne, Danielle F. de Magalhães-Soares, Julia A.G. Silveira, Ricardo A. Machado-de-Ávila, Antônio L. Teixeira, Eduardo A.F. Coelho

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The diagnosis of visceral leishmaniasis (VL) presents problems due to the toxicity and/or high cost of drugs. In addition, no vaccine exists to protect against human disease. In this study, the antigenicity and immunogenicity of amastin protein were evaluated in L. infantum–infected dogs and humans. For the diagnosis, besides the recombinant protein, 1 linear B-cell epitope was synthetized and evaluated in serological assays. Results showed high sensitivity and specificity values to detect the disease when both antigens were employed against a canine and human serological panel. By contrast, when using rA2 and a soluble Leishmania antigenic preparation, sensitivity and specificity values proved to be lower. A preliminary immunogenicity study showed that the amastin protein induced high IFN-γ and low IL-10 production in stimulated PBMC derived from treated VL patients and healthy subjects, thus suggesting a potential use of this protein as an immunogen to protect against human disease.

Original language	English (US)
Pages (from-to)	134-143
Number of pages	10
Journal	Diagnostic Microbiology and Infectious Disease
Volume	95
Issue number	2
DOIs	https://doi.org/10.1016/j.diagmicrobio.2019.04.015
State	Published - Oct 2019
Externally published	Yes

Keywords

Amastin
Diagnosis
Recombinant proteins
Response immune
Synthetic peptides
Visceral leishmaniasis

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

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10.1016/j.diagmicrobio.2019.04.015

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Vale, D. L., Dias, D. S., Machado, A. S., Ribeiro, P. A. F., Lage, D. P., Costa, L. E., Steiner, B. T., Tavares, G. S. V., Ramos, F. F., Martínez-Rodrigo, A., Chávez-Fumagalli, M. A., Caligiorne, R. B., de Magalhães-Soares, D. F., Silveira, J. A. G., Machado-de-Ávila, R. A., Teixeira, A. L., & Coelho, E. A. F. (2019). Diagnostic evaluation of the amastin protein from Leishmania infantum in canine and human visceral leishmaniasis and immunogenicity in human cells derived from patients and healthy controls. Diagnostic Microbiology and Infectious Disease, 95(2), 134-143. https://doi.org/10.1016/j.diagmicrobio.2019.04.015

Diagnostic evaluation of the amastin protein from Leishmania infantum in canine and human visceral leishmaniasis and immunogenicity in human cells derived from patients and healthy controls. / Vale, Danniele L.; Dias, Daniel S.; Machado, Amanda S. et al.
In: Diagnostic Microbiology and Infectious Disease, Vol. 95, No. 2, 10.2019, p. 134-143.

Research output: Contribution to journal › Article › peer-review

Vale, DL, Dias, DS, Machado, AS, Ribeiro, PAF, Lage, DP, Costa, LE, Steiner, BT, Tavares, GSV, Ramos, FF, Martínez-Rodrigo, A, Chávez-Fumagalli, MA, Caligiorne, RB, de Magalhães-Soares, DF, Silveira, JAG, Machado-de-Ávila, RA, Teixeira, AL & Coelho, EAF 2019, 'Diagnostic evaluation of the amastin protein from Leishmania infantum in canine and human visceral leishmaniasis and immunogenicity in human cells derived from patients and healthy controls', Diagnostic Microbiology and Infectious Disease, vol. 95, no. 2, pp. 134-143. https://doi.org/10.1016/j.diagmicrobio.2019.04.015

Vale DL, Dias DS, Machado AS, Ribeiro PAF, Lage DP, Costa LE et al. Diagnostic evaluation of the amastin protein from Leishmania infantum in canine and human visceral leishmaniasis and immunogenicity in human cells derived from patients and healthy controls. Diagnostic Microbiology and Infectious Disease. 2019 Oct;95(2):134-143. doi: 10.1016/j.diagmicrobio.2019.04.015

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title = "Diagnostic evaluation of the amastin protein from Leishmania infantum in canine and human visceral leishmaniasis and immunogenicity in human cells derived from patients and healthy controls",

abstract = "The diagnosis of visceral leishmaniasis (VL) presents problems due to the toxicity and/or high cost of drugs. In addition, no vaccine exists to protect against human disease. In this study, the antigenicity and immunogenicity of amastin protein were evaluated in L. infantum–infected dogs and humans. For the diagnosis, besides the recombinant protein, 1 linear B-cell epitope was synthetized and evaluated in serological assays. Results showed high sensitivity and specificity values to detect the disease when both antigens were employed against a canine and human serological panel. By contrast, when using rA2 and a soluble Leishmania antigenic preparation, sensitivity and specificity values proved to be lower. A preliminary immunogenicity study showed that the amastin protein induced high IFN-γ and low IL-10 production in stimulated PBMC derived from treated VL patients and healthy subjects, thus suggesting a potential use of this protein as an immunogen to protect against human disease.",

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AU - Machado, Amanda S.

AU - Ribeiro, Patrícia A.F.

AU - Lage, Daniela P.

AU - Costa, Lourena E.

AU - Steiner, Bethina T.

AU - Tavares, Grasiele S.V.

AU - Ramos, Fernanda F.

AU - Martínez-Rodrigo, Abel

AU - Chávez-Fumagalli, Miguel A.

AU - Caligiorne, Rachel B.

AU - de Magalhães-Soares, Danielle F.

AU - Silveira, Julia A.G.

AU - Machado-de-Ávila, Ricardo A.

AU - Teixeira, Antônio L.

AU - Coelho, Eduardo A.F.

PY - 2019/10

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N2 - The diagnosis of visceral leishmaniasis (VL) presents problems due to the toxicity and/or high cost of drugs. In addition, no vaccine exists to protect against human disease. In this study, the antigenicity and immunogenicity of amastin protein were evaluated in L. infantum–infected dogs and humans. For the diagnosis, besides the recombinant protein, 1 linear B-cell epitope was synthetized and evaluated in serological assays. Results showed high sensitivity and specificity values to detect the disease when both antigens were employed against a canine and human serological panel. By contrast, when using rA2 and a soluble Leishmania antigenic preparation, sensitivity and specificity values proved to be lower. A preliminary immunogenicity study showed that the amastin protein induced high IFN-γ and low IL-10 production in stimulated PBMC derived from treated VL patients and healthy subjects, thus suggesting a potential use of this protein as an immunogen to protect against human disease.

AB - The diagnosis of visceral leishmaniasis (VL) presents problems due to the toxicity and/or high cost of drugs. In addition, no vaccine exists to protect against human disease. In this study, the antigenicity and immunogenicity of amastin protein were evaluated in L. infantum–infected dogs and humans. For the diagnosis, besides the recombinant protein, 1 linear B-cell epitope was synthetized and evaluated in serological assays. Results showed high sensitivity and specificity values to detect the disease when both antigens were employed against a canine and human serological panel. By contrast, when using rA2 and a soluble Leishmania antigenic preparation, sensitivity and specificity values proved to be lower. A preliminary immunogenicity study showed that the amastin protein induced high IFN-γ and low IL-10 production in stimulated PBMC derived from treated VL patients and healthy subjects, thus suggesting a potential use of this protein as an immunogen to protect against human disease.

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Diagnostic evaluation of the amastin protein from Leishmania infantum in canine and human visceral leishmaniasis and immunogenicity in human cells derived from patients and healthy controls

Abstract

Keywords

ASJC Scopus subject areas

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