Diagnosis of oral hairy leukoplakia by ultrastructural examination of exfoliative cytologic specimens

Frank J. Kratochvil; G. Patrick Riordan; Paul L. Auclair; Michaell A. Huber; Peter J. Krage

doi:10.1016/0030-4220(90)90410-T

Diagnosis of oral hairy leukoplakia by ultrastructural examination of exfoliative cytologic specimens

Frank J. Kratochvil, G. Patrick Riordan, Paul L. Auclair, Michaell A. Huber, Peter J. Krage

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Lingual exfoliative cytologic specimens (scrapings) were obtained from 18 patients positive for human immunodeficiency virus with clinical oral hairy leukoplakia. Buccal mucosal scrapings were obtained from 12 of these patients. The specimens were processed for examination by transmission electron microscopy (TEM). Sixteen (89%) of the lingual specimens revealed infection of keratinocytes by herpes-type virus. There was no evidence of virus infection in the 12 buccal mucosal scrapings. Fungal hyphae were seen by TEM in 14 (78%) of the lingual scrapings and two (17%) of the buccal scrapings. One exfoliative specimen and two biopsy specimens were stained for Epstein-Barr virus DNA with a DNA probe. The demonstration of herpes-type virions by TEM in keratinocytes from a lesion clinically suspected to be hairy leukoplakia provides direct, objective diagnosis. Furthermore, use of exfoliative cytologic specimens provides a clinically simple, noninvasive technique.

Original language	English (US)
Pages (from-to)	613-618
Number of pages	6
Journal	Oral Surgery, Oral Medicine, Oral Pathology
Volume	70
Issue number	5
DOIs	https://doi.org/10.1016/0030-4220(90)90410-T
State	Published - Nov 1990
Externally published	Yes

ASJC Scopus subject areas

Pathology and Forensic Medicine
Dentistry(all)

Access to Document

10.1016/0030-4220(90)90410-T

Cite this

@article{10a3c1c36e984fe1a1130a2c81b2176e,

title = "Diagnosis of oral hairy leukoplakia by ultrastructural examination of exfoliative cytologic specimens",

abstract = "Lingual exfoliative cytologic specimens (scrapings) were obtained from 18 patients positive for human immunodeficiency virus with clinical oral hairy leukoplakia. Buccal mucosal scrapings were obtained from 12 of these patients. The specimens were processed for examination by transmission electron microscopy (TEM). Sixteen (89%) of the lingual specimens revealed infection of keratinocytes by herpes-type virus. There was no evidence of virus infection in the 12 buccal mucosal scrapings. Fungal hyphae were seen by TEM in 14 (78%) of the lingual scrapings and two (17%) of the buccal scrapings. One exfoliative specimen and two biopsy specimens were stained for Epstein-Barr virus DNA with a DNA probe. The demonstration of herpes-type virions by TEM in keratinocytes from a lesion clinically suspected to be hairy leukoplakia provides direct, objective diagnosis. Furthermore, use of exfoliative cytologic specimens provides a clinically simple, noninvasive technique.",

author = "Kratochvil, {Frank J.} and Riordan, {G. Patrick} and Auclair, {Paul L.} and Huber, {Michaell A.} and Krage, {Peter J.}",

year = "1990",

month = nov,

doi = "10.1016/0030-4220(90)90410-T",

language = "English (US)",

volume = "70",

pages = "613--618",

journal = "Oral Surgery Oral Medicine and Oral Pathology",

issn = "2212-4403",

publisher = "Elsevier USA",

number = "5",

}

TY - JOUR

T1 - Diagnosis of oral hairy leukoplakia by ultrastructural examination of exfoliative cytologic specimens

AU - Kratochvil, Frank J.

AU - Riordan, G. Patrick

AU - Auclair, Paul L.

AU - Huber, Michaell A.

AU - Krage, Peter J.

PY - 1990/11

Y1 - 1990/11

N2 - Lingual exfoliative cytologic specimens (scrapings) were obtained from 18 patients positive for human immunodeficiency virus with clinical oral hairy leukoplakia. Buccal mucosal scrapings were obtained from 12 of these patients. The specimens were processed for examination by transmission electron microscopy (TEM). Sixteen (89%) of the lingual specimens revealed infection of keratinocytes by herpes-type virus. There was no evidence of virus infection in the 12 buccal mucosal scrapings. Fungal hyphae were seen by TEM in 14 (78%) of the lingual scrapings and two (17%) of the buccal scrapings. One exfoliative specimen and two biopsy specimens were stained for Epstein-Barr virus DNA with a DNA probe. The demonstration of herpes-type virions by TEM in keratinocytes from a lesion clinically suspected to be hairy leukoplakia provides direct, objective diagnosis. Furthermore, use of exfoliative cytologic specimens provides a clinically simple, noninvasive technique.

AB - Lingual exfoliative cytologic specimens (scrapings) were obtained from 18 patients positive for human immunodeficiency virus with clinical oral hairy leukoplakia. Buccal mucosal scrapings were obtained from 12 of these patients. The specimens were processed for examination by transmission electron microscopy (TEM). Sixteen (89%) of the lingual specimens revealed infection of keratinocytes by herpes-type virus. There was no evidence of virus infection in the 12 buccal mucosal scrapings. Fungal hyphae were seen by TEM in 14 (78%) of the lingual scrapings and two (17%) of the buccal scrapings. One exfoliative specimen and two biopsy specimens were stained for Epstein-Barr virus DNA with a DNA probe. The demonstration of herpes-type virions by TEM in keratinocytes from a lesion clinically suspected to be hairy leukoplakia provides direct, objective diagnosis. Furthermore, use of exfoliative cytologic specimens provides a clinically simple, noninvasive technique.

UR - http://www.scopus.com/inward/record.url?scp=0025135090&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025135090&partnerID=8YFLogxK

U2 - 10.1016/0030-4220(90)90410-T

DO - 10.1016/0030-4220(90)90410-T

M3 - Article

C2 - 2172887

AN - SCOPUS:0025135090

VL - 70

SP - 613

EP - 618

JO - Oral Surgery Oral Medicine and Oral Pathology

JF - Oral Surgery Oral Medicine and Oral Pathology

SN - 2212-4403

IS - 5

ER -

Diagnosis of oral hairy leukoplakia by ultrastructural examination of exfoliative cytologic specimens

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this