Abstract
Lingual exfoliative cytologic specimens (scrapings) were obtained from 18 patients positive for human immunodeficiency virus with clinical oral hairy leukoplakia. Buccal mucosal scrapings were obtained from 12 of these patients. The specimens were processed for examination by transmission electron microscopy (TEM). Sixteen (89%) of the lingual specimens revealed infection of keratinocytes by herpes-type virus. There was no evidence of virus infection in the 12 buccal mucosal scrapings. Fungal hyphae were seen by TEM in 14 (78%) of the lingual scrapings and two (17%) of the buccal scrapings. One exfoliative specimen and two biopsy specimens were stained for Epstein-Barr virus DNA with a DNA probe. The demonstration of herpes-type virions by TEM in keratinocytes from a lesion clinically suspected to be hairy leukoplakia provides direct, objective diagnosis. Furthermore, use of exfoliative cytologic specimens provides a clinically simple, noninvasive technique.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 613-618 |
| Number of pages | 6 |
| Journal | Oral Surgery, Oral Medicine, Oral Pathology |
| Volume | 70 |
| Issue number | 5 |
| DOIs | |
| State | Published - 1990 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Dentistry(all)
Cite this
Diagnosis of oral hairy leukoplakia by ultrastructural examination of exfoliative cytologic specimens. / Kratochvil, Frank J.; Riordan, G. Patrick; Auclair, Paul L.; Huber, Michaell A; Krage, Peter J.
In: Oral Surgery, Oral Medicine, Oral Pathology, Vol. 70, No. 5, 1990, p. 613-618.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Diagnosis of oral hairy leukoplakia by ultrastructural examination of exfoliative cytologic specimens
AU - Kratochvil, Frank J.
AU - Riordan, G. Patrick
AU - Auclair, Paul L.
AU - Huber, Michaell A
AU - Krage, Peter J.
PY - 1990
Y1 - 1990
N2 - Lingual exfoliative cytologic specimens (scrapings) were obtained from 18 patients positive for human immunodeficiency virus with clinical oral hairy leukoplakia. Buccal mucosal scrapings were obtained from 12 of these patients. The specimens were processed for examination by transmission electron microscopy (TEM). Sixteen (89%) of the lingual specimens revealed infection of keratinocytes by herpes-type virus. There was no evidence of virus infection in the 12 buccal mucosal scrapings. Fungal hyphae were seen by TEM in 14 (78%) of the lingual scrapings and two (17%) of the buccal scrapings. One exfoliative specimen and two biopsy specimens were stained for Epstein-Barr virus DNA with a DNA probe. The demonstration of herpes-type virions by TEM in keratinocytes from a lesion clinically suspected to be hairy leukoplakia provides direct, objective diagnosis. Furthermore, use of exfoliative cytologic specimens provides a clinically simple, noninvasive technique.
AB - Lingual exfoliative cytologic specimens (scrapings) were obtained from 18 patients positive for human immunodeficiency virus with clinical oral hairy leukoplakia. Buccal mucosal scrapings were obtained from 12 of these patients. The specimens were processed for examination by transmission electron microscopy (TEM). Sixteen (89%) of the lingual specimens revealed infection of keratinocytes by herpes-type virus. There was no evidence of virus infection in the 12 buccal mucosal scrapings. Fungal hyphae were seen by TEM in 14 (78%) of the lingual scrapings and two (17%) of the buccal scrapings. One exfoliative specimen and two biopsy specimens were stained for Epstein-Barr virus DNA with a DNA probe. The demonstration of herpes-type virions by TEM in keratinocytes from a lesion clinically suspected to be hairy leukoplakia provides direct, objective diagnosis. Furthermore, use of exfoliative cytologic specimens provides a clinically simple, noninvasive technique.
UR - http://www.scopus.com/inward/record.url?scp=0025135090&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025135090&partnerID=8YFLogxK
U2 - 10.1016/0030-4220(90)90410-T
DO - 10.1016/0030-4220(90)90410-T
M3 - Article
C2 - 2172887
AN - SCOPUS:0025135090
VL - 70
SP - 613
EP - 618
JO - Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
JF - Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
SN - 2212-4403
IS - 5
ER -