Developmental regulation of rat brain/Hep G2 glucose transporter gene expression

H. Werner, Martin L Adamo, W. L. Lowe, C. T. Roberts, D. LeRoith

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

The developmental regulation of rat brain-derived/Hep G2 glucose transporter gene expression was studied by means of Northern blot hybridization, using a rat brain glucose transporter cDNA probe, in order to directly quantify steady state glucose transporter mRNA levels. The results obtained showed different tissue-specific patterns of glucose transporter mRNA levels during ontogenesis; while in brain there was a sustained increase in the levels of the message from 20 days embryogenesis until 50 days postnatal, other organs such as heart, lung, liver, and muscle expressed maximal levels of the glucose transporter mRNA in 20-day fetuses and 1-day neonates, decreasing subsequently to very low levels. The relative expression of the glucose transporter mRNA in the different tissues, at both fetal and adult stages, was analyzed using a solution hybridization-RNase protection assay. This approach revealed that, while the heart expresses the highest levels of glucose transporter mRNA at 20 days of fetal life, the brain shows the highest levels at the adult stage. These results indicate a tissue-specific ontogenic pattern of glucose transporter gene expression, suggesting a developmental role for this glucose transporter gene product.

Original languageEnglish (US)
Pages (from-to)273-279
Number of pages7
JournalMolecular Endocrinology
Volume3
Issue number2
StatePublished - 1989
Externally publishedYes

Fingerprint

Facilitative Glucose Transport Proteins
Gene Expression
Brain
Messenger RNA
Ribonucleases
Northern Blotting
Embryonic Development
Fetus
Complementary DNA
Muscles
Lung
Liver

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Werner, H., Adamo, M. L., Lowe, W. L., Roberts, C. T., & LeRoith, D. (1989). Developmental regulation of rat brain/Hep G2 glucose transporter gene expression. Molecular Endocrinology, 3(2), 273-279.

Developmental regulation of rat brain/Hep G2 glucose transporter gene expression. / Werner, H.; Adamo, Martin L; Lowe, W. L.; Roberts, C. T.; LeRoith, D.

In: Molecular Endocrinology, Vol. 3, No. 2, 1989, p. 273-279.

Research output: Contribution to journalArticle

Werner, H, Adamo, ML, Lowe, WL, Roberts, CT & LeRoith, D 1989, 'Developmental regulation of rat brain/Hep G2 glucose transporter gene expression', Molecular Endocrinology, vol. 3, no. 2, pp. 273-279.
Werner H, Adamo ML, Lowe WL, Roberts CT, LeRoith D. Developmental regulation of rat brain/Hep G2 glucose transporter gene expression. Molecular Endocrinology. 1989;3(2):273-279.
Werner, H. ; Adamo, Martin L ; Lowe, W. L. ; Roberts, C. T. ; LeRoith, D. / Developmental regulation of rat brain/Hep G2 glucose transporter gene expression. In: Molecular Endocrinology. 1989 ; Vol. 3, No. 2. pp. 273-279.
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