Developmental regulation of neuregulin1 isoforms and erbb receptor expression in intact rat dorsal root ganglia

Sara Reinhard, Eric Vela, Nicole Bombara, George H. DeVries, Timothy D. Raabe

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Neuregulins (NRGs) are a family of growth factors which bind to the erbB family of tyrosine kinase receptors. The exact nature and interaction of specific NRG isoforms and erbB receptors that occur during the development of the nervous system have not been reported. In order to better understand the role that different NRG isoforms and erbB receptors play in the differentiation, proliferation, and survival of neurons and glial cells, we isolated protein and mRNA from dorsal root ganglia of rat pups between embryonic day (E) 13 and postnatal day (P) 15. The relative expression levels of the NRGs and erbB receptors for the different time points were compared using both Western and RT-PCR analyses. NRG1-type1α protein levels were highest at E-13 and then decreased by approximately 40% and remained constant through P-15. In contrast, mRNA levels for NRG1-type1α remained constant from E-15 to P-15. The protein levels for NRG1-type 1β were similar to NRG1-type1α at E-13 with an approximate 40% increase in the levels at E-15 and E-17 followed by a decrease to E-13 levels for the remainder of the developmental time periods. The mRNA levels for NRG1-type1β remained constant from E-15 to P-15. The protein and mRNA expression patterns for each erbB receptor were distinctive. The protein levels for erbB-2 were highest at E-19 while erbB-3 levels were highest at E-17 and E-18. ErbB-4 protein levels were highest at E-13 and decreased through P-15. The developmental pattern for erbB-2 and erbB-4 mRNA levels had no relation to that of the corresponding protein levels while the mRNA levels for erbB-3 were highest at E-17 and E-18 similar to the pattern observed for the erbB-3 protein levels. We concluded that both NRG and erbB expression in dorsal root ganglia are mostly translationally controlled and that NRG1 isoforms and their erbB receptors are not coordinately regulated.

Original languageEnglish (US)
Pages (from-to)17-22
Number of pages6
JournalNeurochemical Research
Volume34
Issue number1
DOIs
StatePublished - Jan 2009
Externally publishedYes

Keywords

  • Dorsal root ganglia
  • ErbB-2
  • ErbB-3
  • ErbB-4
  • Neuregulin1-type 1α
  • Neuregulin1-type 1β

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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