Developmental patterns of PPAR and RXR gene expression during spermatogenesis

Kelwyn Thomas, Dae Yong Sung, Xing Chen, Winston Thompson, Y. Eugene Chen, John McCarrey, William Walker, Michael Griswold

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


Members of the family of nuclear receptors that include peroxisome proliferator-activated receptors (PPARs) and retinoid X receptors (RXRs) are important mediators of selective gene activation during development and cellular differentiation. In this study, developmentallyspecific PPAR and RXR patterns of expression that occur in somatic and germ cell populations in the testis were determined using quantative real-time PCR (qRT-PCR) studies on RNAs that were isolated from StaPut purified mouse germ cells and primary rat Sertoli cells. These qRT-PCR studies indicate that transcripts encoding the PPAR-Alpha (α), -Beta (β), and-Gamma (γ) and RXR - Alpha (α), -Beta (β), and -Gamma (γ) are developmentally expressed in both differentiating germ and Sertoli cells. In further experiments aimed at deciphering the physiological role that PPAR-Gamma (γ) plays in Sertoli cells, 15-day primary rat Sertoli cells were infected with recombinant adenoviral vectors containing PPAR-Gamma (γ) cDNA and PPAR-Gamma (γ) RNAi constructs. Affymetrix microarray analysis and qRT-PCR validation studies using total RNA isolated from these transfected cells indicated that PPAR-Gamma (γ) regulates the pattern of expression of key lipid metabolic genes in Sertoli cells.

Original languageEnglish (US)
Pages (from-to)1209-1220
Number of pages12
JournalFrontiers in Bioscience - Elite
Volume3 E
Issue number4
StatePublished - Jan 6 2011
Externally publishedYes


  • Gene expression
  • Nuclear receptors
  • Spermatogenesis
  • Testis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)


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