Developmental decisions in Aspergillus nidulans are modulated by Ras activity

Tapan Som, Venkata S R Kolaparthi

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

To better understand how Ras controls development of multicellular organisms, we have chosen Aspergillus nidulans as a model system. When grown on solid medium, this fungus follows a well-defined program of development, sequentially giving rise to several cell types which produce three distinct structures: vegetative hyphae, aerial hyphae, and the conidiophore structure. Here we describe a ras homolog found in this fungus (Aras) and demonstrate that it is an essential gene that regulates the ordered program of development. We created dominant alleles of this gene and expressed them to different levels in order to vary the ratio of GTP-bound (active) to GDP- bound (inactive) A-Ras protein. When the amount of active Ras is large, nuclear division proceeds, but further development is inhibited at the early step of germ tube formation. At an intermediate level of active Ras, aerial hypha formation is inhibited, while at a low level, conidiophore formation is inhibited. Maintenance of an even lower level of the active Ras is essential for initiation and progression of conidiophore formation, the final stage of development. When the level of active Ras is artificially lowered, each stage of development is initiated prematurely except germination, the initial stage of development. Therefore, the progression of the ordered developmental pathway of A. nidulans is dependent upon an initial high level of active Ras followed by its gradual decrease. We propose that several concentration thresholds exist, each of which allows development to proceed to a certain point, producing the proper cell type while inhibiting further development.

Original languageEnglish (US)
Pages (from-to)5333-5348
Number of pages16
JournalMolecular and Cellular Biology
Volume14
Issue number8
StatePublished - Aug 1994
Externally publishedYes

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Aspergillus nidulans
Hyphae
Program Development
Fungi
Cell Nucleus Division
Dominant Genes
ras Proteins
Essential Genes
Germination
Guanosine Triphosphate
Alleles
Maintenance

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Developmental decisions in Aspergillus nidulans are modulated by Ras activity. / Som, Tapan; Kolaparthi, Venkata S R.

In: Molecular and Cellular Biology, Vol. 14, No. 8, 08.1994, p. 5333-5348.

Research output: Contribution to journalArticle

Som, Tapan ; Kolaparthi, Venkata S R. / Developmental decisions in Aspergillus nidulans are modulated by Ras activity. In: Molecular and Cellular Biology. 1994 ; Vol. 14, No. 8. pp. 5333-5348.
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