Developmental changes of beta-adrenergic receptor-linked adenylate cyclase of rat liver.

M. S. Katz, S. R. Boland, S. J. Schmidt

Research output: Contribution to journalArticle

Abstract

beta-Adrenergic agonist-sensitive adenylate cyclase activity and binding of the beta-adrenergic antagonist(-)-[125I]iodopindolol were studied in rat liver during development of male Fischer 344 rats ages 6-60 days. In liver homogenates maximum adenylate cyclase response to beta-adrenergic agonist (10(-5) M isoproterenol or epinephrine) decreased by 73% (P less than 0.01) between 6 and 60 days, with most of the decrease (56%; P less than 0.01) occurring by 20 days. beta-adrenergic receptor density (Bmax) showed a corresponding decrease of 66% (P less than 0.01) by 20 days without subsequent change. Binding characteristics of stereospecificity, pharmacological specificity, saturability with time, and reversibility were unchanged with age. GTP-, fluoride-, forskolin-, and Mn2+-stimulated adenylate cyclase activities also decreased during development, suggesting a decrease of activity of the catalytic component and/or guanine nucleotide regulatory component of adenylate cyclase. These results indicate that the developmental decrease of beta-adrenergic agonist-sensitive adenylate cyclase activity may result from decreased numbers of beta-adrenergic receptors. Developmental alterations of nonreceptor components of the enzyme may also contribute to changes of catecholamine-sensitive adenylate cyclase.

Original languageEnglish (US)
JournalThe American journal of physiology
Volume248
Issue number6 Pt 1
StatePublished - Jun 1985
Externally publishedYes

Fingerprint

Receptors, Adrenergic, beta
Adenylyl Cyclases
Adrenergic beta-Agonists
Liver
Adrenergic beta-Antagonists
Guanine Nucleotides
Inbred F344 Rats
Colforsin
Guanosine Triphosphate
Fluorides
Isoproterenol
Epinephrine
Catecholamines
Pharmacology
Enzymes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Developmental changes of beta-adrenergic receptor-linked adenylate cyclase of rat liver. / Katz, M. S.; Boland, S. R.; Schmidt, S. J.

In: The American journal of physiology, Vol. 248, No. 6 Pt 1, 06.1985.

Research output: Contribution to journalArticle

Katz, M. S. ; Boland, S. R. ; Schmidt, S. J. / Developmental changes of beta-adrenergic receptor-linked adenylate cyclase of rat liver. In: The American journal of physiology. 1985 ; Vol. 248, No. 6 Pt 1.
@article{000d6eb188684afc9acbb4da52d7f946,
title = "Developmental changes of beta-adrenergic receptor-linked adenylate cyclase of rat liver.",
abstract = "beta-Adrenergic agonist-sensitive adenylate cyclase activity and binding of the beta-adrenergic antagonist(-)-[125I]iodopindolol were studied in rat liver during development of male Fischer 344 rats ages 6-60 days. In liver homogenates maximum adenylate cyclase response to beta-adrenergic agonist (10(-5) M isoproterenol or epinephrine) decreased by 73{\%} (P less than 0.01) between 6 and 60 days, with most of the decrease (56{\%}; P less than 0.01) occurring by 20 days. beta-adrenergic receptor density (Bmax) showed a corresponding decrease of 66{\%} (P less than 0.01) by 20 days without subsequent change. Binding characteristics of stereospecificity, pharmacological specificity, saturability with time, and reversibility were unchanged with age. GTP-, fluoride-, forskolin-, and Mn2+-stimulated adenylate cyclase activities also decreased during development, suggesting a decrease of activity of the catalytic component and/or guanine nucleotide regulatory component of adenylate cyclase. These results indicate that the developmental decrease of beta-adrenergic agonist-sensitive adenylate cyclase activity may result from decreased numbers of beta-adrenergic receptors. Developmental alterations of nonreceptor components of the enzyme may also contribute to changes of catecholamine-sensitive adenylate cyclase.",
author = "Katz, {M. S.} and Boland, {S. R.} and Schmidt, {S. J.}",
year = "1985",
month = "6",
language = "English (US)",
volume = "248",
journal = "American Journal of Physiology - Renal Physiology",
issn = "0363-6127",
publisher = "American Physiological Society",
number = "6 Pt 1",

}

TY - JOUR

T1 - Developmental changes of beta-adrenergic receptor-linked adenylate cyclase of rat liver.

AU - Katz, M. S.

AU - Boland, S. R.

AU - Schmidt, S. J.

PY - 1985/6

Y1 - 1985/6

N2 - beta-Adrenergic agonist-sensitive adenylate cyclase activity and binding of the beta-adrenergic antagonist(-)-[125I]iodopindolol were studied in rat liver during development of male Fischer 344 rats ages 6-60 days. In liver homogenates maximum adenylate cyclase response to beta-adrenergic agonist (10(-5) M isoproterenol or epinephrine) decreased by 73% (P less than 0.01) between 6 and 60 days, with most of the decrease (56%; P less than 0.01) occurring by 20 days. beta-adrenergic receptor density (Bmax) showed a corresponding decrease of 66% (P less than 0.01) by 20 days without subsequent change. Binding characteristics of stereospecificity, pharmacological specificity, saturability with time, and reversibility were unchanged with age. GTP-, fluoride-, forskolin-, and Mn2+-stimulated adenylate cyclase activities also decreased during development, suggesting a decrease of activity of the catalytic component and/or guanine nucleotide regulatory component of adenylate cyclase. These results indicate that the developmental decrease of beta-adrenergic agonist-sensitive adenylate cyclase activity may result from decreased numbers of beta-adrenergic receptors. Developmental alterations of nonreceptor components of the enzyme may also contribute to changes of catecholamine-sensitive adenylate cyclase.

AB - beta-Adrenergic agonist-sensitive adenylate cyclase activity and binding of the beta-adrenergic antagonist(-)-[125I]iodopindolol were studied in rat liver during development of male Fischer 344 rats ages 6-60 days. In liver homogenates maximum adenylate cyclase response to beta-adrenergic agonist (10(-5) M isoproterenol or epinephrine) decreased by 73% (P less than 0.01) between 6 and 60 days, with most of the decrease (56%; P less than 0.01) occurring by 20 days. beta-adrenergic receptor density (Bmax) showed a corresponding decrease of 66% (P less than 0.01) by 20 days without subsequent change. Binding characteristics of stereospecificity, pharmacological specificity, saturability with time, and reversibility were unchanged with age. GTP-, fluoride-, forskolin-, and Mn2+-stimulated adenylate cyclase activities also decreased during development, suggesting a decrease of activity of the catalytic component and/or guanine nucleotide regulatory component of adenylate cyclase. These results indicate that the developmental decrease of beta-adrenergic agonist-sensitive adenylate cyclase activity may result from decreased numbers of beta-adrenergic receptors. Developmental alterations of nonreceptor components of the enzyme may also contribute to changes of catecholamine-sensitive adenylate cyclase.

UR - http://www.scopus.com/inward/record.url?scp=0022081152&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022081152&partnerID=8YFLogxK

M3 - Article

C2 - 2860808

VL - 248

JO - American Journal of Physiology - Renal Physiology

JF - American Journal of Physiology - Renal Physiology

SN - 0363-6127

IS - 6 Pt 1

ER -