Developmental changes in methylation of spermatogenesis–specific genes include reprogramming in the epididymis

Mira Ariel, Howard Cedar, John McCarrey

Research output: Contribution to journalArticlepeer-review

143 Scopus citations

Abstract

We have determined the status of DNA methylation at specific sites in three spermatogenesis–specific genes, Pgk–2, ApoA1 and Oct–3/4, throughout the development and differentiation of male germ cells in the mouse. We observed a specific demethylation event in the Pgk–2 gene in prospermatogonia at about the time of birth, about 10 days before the onset of transcription which first occurs in primary spermatocytes. All three genes were unmethylated in adult spermatogenic cells in the testis, but were remethylated in mature spermatozoa in the vas deferens. Surprisingly, we found that this remethylation is part of the process of sperm maturation which occurs in the epididymis.

Original languageEnglish (US)
Pages (from-to)59-63
Number of pages5
JournalNature Genetics
Volume7
Issue number1
DOIs
StatePublished - May 1994
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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