Developmental aspects of androgen-dependent mRNA from rat ventral prostate using cloned cDNA

Donald B. Carter, Koji Yamada, Stephen E. Harris

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


The androgen-dependence of two mRNAs from rat ventral prostate coding for a 20000 and an 11000 dalton translation product has been investigated using complementary DNA cloned in the bacterial plasmid PBR322. One of the cloned insert DNAs from a recombinant plasmid, C-27, arrests the in vitro translation of C2 (Peeters et al. (1980) J. Biol. Chem. 255, 7017-7023). The other cloned insert DNA arrests the translation of a glycoprotein 20000 dallons in size, with unknown function. The quantities of mRNA coding for the 20000 and 11000 dalton translation product were determined by hybridization of 32P-labeled inserts to filter-bound total RNA or poly(A+)-mRNA. Castration caused a decline in both mRNAs of 250-fold over 8 days. Stimulation with androgen of 5-week castrates restored the mRNA levels to 17% of intact for the 20000 dalton translation product and 31% of intact for the 11000 dalton translation product. The quantity of the two mRNAs found in the lateral poly(A+)-mRNA was about 1/10 that of the ventral level and the mRNAs were not detectable in the dorsal prostate, seminal vesicle or human prostate poly(A+)-mRNA populations. RNA from the ventral prostates of animals 10-21 days old contained mature levels of complementary sequences, suggesting a form of developmental posttranscriptional regulation for synthesis of the polypeptides which are not synthesized in mature quantities at this stage of development (Heyns et al. (1978) Endocrinology 103, 1090-1095; Kistler et al. (1981) Proc. Natl. Acad. Sci. (U.S.A.) 78, 737-741).

Original languageEnglish (US)
Pages (from-to)199-214
Number of pages16
JournalMolecular and Cellular Endocrinology
Issue number2-3
StatePublished - Aug 1983
Externally publishedYes


  • dot-hybridization
  • ontogeny of abundant mRNA
  • prostatic binding protein
  • restriction maps

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology


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