Development pattern of the GABAA-benzodiazepine receptor ionophore complex in primary cultures of cortical neurons

Xian Jue Hu, Maharaj K. Ticku

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The development pattern of the GABAA/benzodiazepine (BZ) receptor ionophore complex was characterized in mammalian cortical cultured neurons utilizing radioligand binding and GABA-induced 36Cl-influx. The specific binding of [3H]flunitrazepam, [3H]Ro15-1788 and [3H]Ro15-4513 increased with time from day 3 to day 21. The EC50 value and Emax of the GABA, muscimol and pentobarbital to enhance [3H]-flunitrazepam binding did not change during the development. Furthermore, GABA-induced 36Cl- influx also did not change during development from day 3 to day 21. However, the potency of ligands that bind to type-1 BZ receptors (alprazolam, zolpidem and C1218,872) to inhibit [3H]flunitrazepam binding increased from day 7 to day 21. Taken together, these data suggest the presence of type-II BZ receptors in early stages of the development which appears to change to type-I BZ receptors with age in the cortical neurons in culture.

Original languageEnglish (US)
Pages (from-to)137-140
Number of pages4
JournalDevelopmental Brain Research
Volume80
Issue number1-2
DOIs
StatePublished - Jul 15 1994
Externally publishedYes

Keywords

  • Cl influx
  • Cortical cultured neuron
  • GABA-BZ receptor ionophore complex

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Developmental Biology

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