Development of metabolic function biomarkers in the common marmoset, callithrix jacchus

Toni E. Ziegler, Ricki J. Colman, Suzette D. Tardif, Megan E. Sosa, Fredrick H. Wegner, Daniel J. Wittwer, Hemanta Shrestha

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Metabolic assessment of a non-human primate model of metabolic syndrome and obesity requires the necessary biomarkers specific to the species. While the rhesus monkey has a number of specific assays for assessing metabolic syndrome, the marmoset does not. Furthermore, the common marmoset (Callithrix jacchus) has a small blood volume that necessitates using a single blood volume for multiple analyses. The common marmoset holds a great potential as an alternative primate model for the study of human disease but assay methods need to be developed and validated for the biomarkers of metabolic syndrome. Here we report on the adaptation, development, and validation of commercially available immunoassays for common marmoset samples in small volumes. We have performed biological validations for insulin, adiponectin, leptin, and ghrelin to demonstrate the use of these biomarkers in examining metabolic syndrome and other related diseases in the common marmoset. Am. J. Primatol. 75:500-508, 2013.

Original languageEnglish (US)
Pages (from-to)500-508
Number of pages9
JournalAmerican Journal of Primatology
Volume75
Issue number5
DOIs
StatePublished - May 2013

Fingerprint

Callithrix jacchus
metabolic syndrome
biomarker
biomarkers
primate
blood
assay
blood volume
obesity
immunoassay
animal models
ghrelin
adiponectin
Callitrichidae
assays
Macaca mulatta
leptin
immunoassays
human diseases
insulin

Keywords

  • Adiponectin
  • Ghrelin
  • Insulin
  • Leptin
  • Marmoset
  • Metabolic syndrome

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Ziegler, T. E., Colman, R. J., Tardif, S. D., Sosa, M. E., Wegner, F. H., Wittwer, D. J., & Shrestha, H. (2013). Development of metabolic function biomarkers in the common marmoset, callithrix jacchus. American Journal of Primatology, 75(5), 500-508. https://doi.org/10.1002/ajp.22126

Development of metabolic function biomarkers in the common marmoset, callithrix jacchus. / Ziegler, Toni E.; Colman, Ricki J.; Tardif, Suzette D.; Sosa, Megan E.; Wegner, Fredrick H.; Wittwer, Daniel J.; Shrestha, Hemanta.

In: American Journal of Primatology, Vol. 75, No. 5, 05.2013, p. 500-508.

Research output: Contribution to journalArticle

Ziegler, TE, Colman, RJ, Tardif, SD, Sosa, ME, Wegner, FH, Wittwer, DJ & Shrestha, H 2013, 'Development of metabolic function biomarkers in the common marmoset, callithrix jacchus', American Journal of Primatology, vol. 75, no. 5, pp. 500-508. https://doi.org/10.1002/ajp.22126
Ziegler, Toni E. ; Colman, Ricki J. ; Tardif, Suzette D. ; Sosa, Megan E. ; Wegner, Fredrick H. ; Wittwer, Daniel J. ; Shrestha, Hemanta. / Development of metabolic function biomarkers in the common marmoset, callithrix jacchus. In: American Journal of Primatology. 2013 ; Vol. 75, No. 5. pp. 500-508.
@article{47a9df2be6b1415891b464feb88227ec,
title = "Development of metabolic function biomarkers in the common marmoset, callithrix jacchus",
abstract = "Metabolic assessment of a non-human primate model of metabolic syndrome and obesity requires the necessary biomarkers specific to the species. While the rhesus monkey has a number of specific assays for assessing metabolic syndrome, the marmoset does not. Furthermore, the common marmoset (Callithrix jacchus) has a small blood volume that necessitates using a single blood volume for multiple analyses. The common marmoset holds a great potential as an alternative primate model for the study of human disease but assay methods need to be developed and validated for the biomarkers of metabolic syndrome. Here we report on the adaptation, development, and validation of commercially available immunoassays for common marmoset samples in small volumes. We have performed biological validations for insulin, adiponectin, leptin, and ghrelin to demonstrate the use of these biomarkers in examining metabolic syndrome and other related diseases in the common marmoset. Am. J. Primatol. 75:500-508, 2013.",
keywords = "Adiponectin, Ghrelin, Insulin, Leptin, Marmoset, Metabolic syndrome",
author = "Ziegler, {Toni E.} and Colman, {Ricki J.} and Tardif, {Suzette D.} and Sosa, {Megan E.} and Wegner, {Fredrick H.} and Wittwer, {Daniel J.} and Hemanta Shrestha",
year = "2013",
month = "5",
doi = "10.1002/ajp.22126",
language = "English (US)",
volume = "75",
pages = "500--508",
journal = "American Journal of Primatology",
issn = "0275-2565",
publisher = "John Wiley and Sons Inc.",
number = "5",

}

TY - JOUR

T1 - Development of metabolic function biomarkers in the common marmoset, callithrix jacchus

AU - Ziegler, Toni E.

AU - Colman, Ricki J.

AU - Tardif, Suzette D.

AU - Sosa, Megan E.

AU - Wegner, Fredrick H.

AU - Wittwer, Daniel J.

AU - Shrestha, Hemanta

PY - 2013/5

Y1 - 2013/5

N2 - Metabolic assessment of a non-human primate model of metabolic syndrome and obesity requires the necessary biomarkers specific to the species. While the rhesus monkey has a number of specific assays for assessing metabolic syndrome, the marmoset does not. Furthermore, the common marmoset (Callithrix jacchus) has a small blood volume that necessitates using a single blood volume for multiple analyses. The common marmoset holds a great potential as an alternative primate model for the study of human disease but assay methods need to be developed and validated for the biomarkers of metabolic syndrome. Here we report on the adaptation, development, and validation of commercially available immunoassays for common marmoset samples in small volumes. We have performed biological validations for insulin, adiponectin, leptin, and ghrelin to demonstrate the use of these biomarkers in examining metabolic syndrome and other related diseases in the common marmoset. Am. J. Primatol. 75:500-508, 2013.

AB - Metabolic assessment of a non-human primate model of metabolic syndrome and obesity requires the necessary biomarkers specific to the species. While the rhesus monkey has a number of specific assays for assessing metabolic syndrome, the marmoset does not. Furthermore, the common marmoset (Callithrix jacchus) has a small blood volume that necessitates using a single blood volume for multiple analyses. The common marmoset holds a great potential as an alternative primate model for the study of human disease but assay methods need to be developed and validated for the biomarkers of metabolic syndrome. Here we report on the adaptation, development, and validation of commercially available immunoassays for common marmoset samples in small volumes. We have performed biological validations for insulin, adiponectin, leptin, and ghrelin to demonstrate the use of these biomarkers in examining metabolic syndrome and other related diseases in the common marmoset. Am. J. Primatol. 75:500-508, 2013.

KW - Adiponectin

KW - Ghrelin

KW - Insulin

KW - Leptin

KW - Marmoset

KW - Metabolic syndrome

UR - http://www.scopus.com/inward/record.url?scp=84875639596&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875639596&partnerID=8YFLogxK

U2 - 10.1002/ajp.22126

DO - 10.1002/ajp.22126

M3 - Article

C2 - 23447060

AN - SCOPUS:84875639596

VL - 75

SP - 500

EP - 508

JO - American Journal of Primatology

JF - American Journal of Primatology

SN - 0275-2565

IS - 5

ER -