Development, inheritance, and linkage-group assignment of 60 novel microsatellite markers for the gray, short-tailed opossum Monodelphis domestica

Nicolas Gouin, Scott J. Westenberger, Susan M. Mahaney, Peter Lindley, John L. VandeBerg, Paul B. Samollow

    Research output: Contribution to journalArticle

    5 Scopus citations

    Abstract

    Short-tandem-repeat (SSR) or microsatellite polymorphisms are some of the most extensively employed genetic markers in contemporary linkage mapping studies. To date, only a limited number of microsatellites have been isolated in the gray, short-tailed opossum Monodelphis domestica, a South American marsupial widely used for comparative biological and biomedical research. To increase the number of potentially useful mapping markers, we screened 2 microsatellite-enriched genomic libraries containing alternatively (CA) n or (GA)n repeats. A total of 184 clones were sequenced, from which 60 polymorphic microsatellite markers were successfully optimized. The efficiency of this enrichment protocol for M. domestica microsatellite isolation is discussed, and suggestions to improve the outcome are made. All 60 loci showed high allelic diversity, with allele numbers ranging from 2 to 10 in a subset of 33 unrelated animals. Normal Mendelian inheritance was confirmed for all loci by analyzing allelic segregation in 5 two-generation families. One microsatellite appeared to be X linked, and null alleles were found in 5 others. Two-point linkage analyses were implemented using the data on the 5 families, leading to the assignment of 59 of these loci to the existing linkage groups. The 60 novel microsatellites developed in this study will contribute significantly to the M. domestica linkage map, and further QTL mapping studies.

    Original languageEnglish (US)
    Pages (from-to)1019-1027
    Number of pages9
    JournalGenome
    Volume48
    Issue number6
    DOIs
    StatePublished - Dec 1 2005

    Keywords

    • Enriched libraries
    • Genetic linkage analysis
    • Marsupial
    • Microsatellite
    • Monodelphis domestica

    ASJC Scopus subject areas

    • Biotechnology
    • Molecular Biology
    • Genetics

    Fingerprint Dive into the research topics of 'Development, inheritance, and linkage-group assignment of 60 novel microsatellite markers for the gray, short-tailed opossum Monodelphis domestica'. Together they form a unique fingerprint.

  • Cite this

    Gouin, N., Westenberger, S. J., Mahaney, S. M., Lindley, P., VandeBerg, J. L., & Samollow, P. B. (2005). Development, inheritance, and linkage-group assignment of 60 novel microsatellite markers for the gray, short-tailed opossum Monodelphis domestica. Genome, 48(6), 1019-1027. https://doi.org/10.1139/g05-059