Abstract
We have developed a model system of late stage metastatic progression by isolating a highly malignant variant of human breast cancer cells from the parental MDA-MB-435 cell line. These cells, isolated from early lung metastasis, displayed increased anchorage independent growth in vitro and when transplanted ortho-topically into nude mice showed accelerated tumor growth rate and early lung spontaneous metastasis when compared to its parental counterpart. These cells, designated as MDA-MB-435-F-L, also showed intense wide spread early skeletal metastasis in vertebrae, mandible, femur, tibia and skull as detected by fluorescence imaging in an experimental bone metastasis model. Gene expression profiles from cDNA microarray showed up or downregulation of the expression of several significant genes regulating angiogenesis, apoptosis, ECM remodeling and metastasis in the MDA-MB-435-F-L cells in comparison to the parental cells. Among the up or downregulated genes, some have also been implicated in the survival of breast cancer patients. As such, the candidate genes selected in this breast cancer progression model system may serve as biomarkers of metastatic progression and also as potential tumor targets for breast cancer therapy.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 168-174 |
| Number of pages | 7 |
| Journal | Cancer Biology and Therapy |
| Volume | 4 |
| Issue number | 2 |
| State | Published - Feb 2005 |
Fingerprint
Keywords
- Bone metastasis
- Breast cancer
- cDNA-microarray
- Green fluorescence protein
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Development and gene expression profiling of a metastatic variant of the human breast cancer MDA-MB-435 cells. / Bandyopadhyay, Abhik; Elkahloun, Abdel; Baysa, Sherrie Joy; Wang, Long; Sun, Luzhe.
In: Cancer Biology and Therapy, Vol. 4, No. 2, 02.2005, p. 168-174.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development and gene expression profiling of a metastatic variant of the human breast cancer MDA-MB-435 cells
AU - Bandyopadhyay, Abhik
AU - Elkahloun, Abdel
AU - Baysa, Sherrie Joy
AU - Wang, Long
AU - Sun, Luzhe
PY - 2005/2
Y1 - 2005/2
N2 - We have developed a model system of late stage metastatic progression by isolating a highly malignant variant of human breast cancer cells from the parental MDA-MB-435 cell line. These cells, isolated from early lung metastasis, displayed increased anchorage independent growth in vitro and when transplanted ortho-topically into nude mice showed accelerated tumor growth rate and early lung spontaneous metastasis when compared to its parental counterpart. These cells, designated as MDA-MB-435-F-L, also showed intense wide spread early skeletal metastasis in vertebrae, mandible, femur, tibia and skull as detected by fluorescence imaging in an experimental bone metastasis model. Gene expression profiles from cDNA microarray showed up or downregulation of the expression of several significant genes regulating angiogenesis, apoptosis, ECM remodeling and metastasis in the MDA-MB-435-F-L cells in comparison to the parental cells. Among the up or downregulated genes, some have also been implicated in the survival of breast cancer patients. As such, the candidate genes selected in this breast cancer progression model system may serve as biomarkers of metastatic progression and also as potential tumor targets for breast cancer therapy.
AB - We have developed a model system of late stage metastatic progression by isolating a highly malignant variant of human breast cancer cells from the parental MDA-MB-435 cell line. These cells, isolated from early lung metastasis, displayed increased anchorage independent growth in vitro and when transplanted ortho-topically into nude mice showed accelerated tumor growth rate and early lung spontaneous metastasis when compared to its parental counterpart. These cells, designated as MDA-MB-435-F-L, also showed intense wide spread early skeletal metastasis in vertebrae, mandible, femur, tibia and skull as detected by fluorescence imaging in an experimental bone metastasis model. Gene expression profiles from cDNA microarray showed up or downregulation of the expression of several significant genes regulating angiogenesis, apoptosis, ECM remodeling and metastasis in the MDA-MB-435-F-L cells in comparison to the parental cells. Among the up or downregulated genes, some have also been implicated in the survival of breast cancer patients. As such, the candidate genes selected in this breast cancer progression model system may serve as biomarkers of metastatic progression and also as potential tumor targets for breast cancer therapy.
KW - Bone metastasis
KW - Breast cancer
KW - cDNA-microarray
KW - Green fluorescence protein
UR - http://www.scopus.com/inward/record.url?scp=25144454435&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=25144454435&partnerID=8YFLogxK
M3 - Article
C2 - 15655343
AN - SCOPUS:25144454435
VL - 4
SP - 168
EP - 174
JO - Cancer Biology and Therapy
JF - Cancer Biology and Therapy
SN - 1538-4047
IS - 2
ER -