Development and gene expression profiling of a metastatic variant of the human breast cancer MDA-MB-435 cells

Abhik Bandyopadhyay, Abdel Elkahloun, Sherrie Joy Baysa, Long Wang, Luzhe Sun

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We have developed a model system of late stage metastatic progression by isolating a highly malignant variant of human breast cancer cells from the parental MDA-MB-435 cell line. These cells, isolated from early lung metastasis, displayed increased anchorage independent growth in vitro and when transplanted ortho-topically into nude mice showed accelerated tumor growth rate and early lung spontaneous metastasis when compared to its parental counterpart. These cells, designated as MDA-MB-435-F-L, also showed intense wide spread early skeletal metastasis in vertebrae, mandible, femur, tibia and skull as detected by fluorescence imaging in an experimental bone metastasis model. Gene expression profiles from cDNA microarray showed up or downregulation of the expression of several significant genes regulating angiogenesis, apoptosis, ECM remodeling and metastasis in the MDA-MB-435-F-L cells in comparison to the parental cells. Among the up or downregulated genes, some have also been implicated in the survival of breast cancer patients. As such, the candidate genes selected in this breast cancer progression model system may serve as biomarkers of metastatic progression and also as potential tumor targets for breast cancer therapy.

Original languageEnglish (US)
Pages (from-to)168-174
Number of pages7
JournalCancer Biology and Therapy
Volume4
Issue number2
StatePublished - Feb 2005

Fingerprint

Gene Expression Profiling
Breast Neoplasms
Neoplasm Metastasis
Down-Regulation
Genes
Lung
Optical Imaging
Growth
Oligonucleotide Array Sequence Analysis
Tibia
Mandible
Transcriptome
Skull
Nude Mice
Femur
Neoplasms
Spine
Biomarkers
Apoptosis
Bone and Bones

Keywords

  • Bone metastasis
  • Breast cancer
  • cDNA-microarray
  • Green fluorescence protein

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Development and gene expression profiling of a metastatic variant of the human breast cancer MDA-MB-435 cells. / Bandyopadhyay, Abhik; Elkahloun, Abdel; Baysa, Sherrie Joy; Wang, Long; Sun, Luzhe.

In: Cancer Biology and Therapy, Vol. 4, No. 2, 02.2005, p. 168-174.

Research output: Contribution to journalArticle

Bandyopadhyay, Abhik ; Elkahloun, Abdel ; Baysa, Sherrie Joy ; Wang, Long ; Sun, Luzhe. / Development and gene expression profiling of a metastatic variant of the human breast cancer MDA-MB-435 cells. In: Cancer Biology and Therapy. 2005 ; Vol. 4, No. 2. pp. 168-174.
@article{fbc6323b4c2c4a43ab3753721e0d4890,
title = "Development and gene expression profiling of a metastatic variant of the human breast cancer MDA-MB-435 cells",
abstract = "We have developed a model system of late stage metastatic progression by isolating a highly malignant variant of human breast cancer cells from the parental MDA-MB-435 cell line. These cells, isolated from early lung metastasis, displayed increased anchorage independent growth in vitro and when transplanted ortho-topically into nude mice showed accelerated tumor growth rate and early lung spontaneous metastasis when compared to its parental counterpart. These cells, designated as MDA-MB-435-F-L, also showed intense wide spread early skeletal metastasis in vertebrae, mandible, femur, tibia and skull as detected by fluorescence imaging in an experimental bone metastasis model. Gene expression profiles from cDNA microarray showed up or downregulation of the expression of several significant genes regulating angiogenesis, apoptosis, ECM remodeling and metastasis in the MDA-MB-435-F-L cells in comparison to the parental cells. Among the up or downregulated genes, some have also been implicated in the survival of breast cancer patients. As such, the candidate genes selected in this breast cancer progression model system may serve as biomarkers of metastatic progression and also as potential tumor targets for breast cancer therapy.",
keywords = "Bone metastasis, Breast cancer, cDNA-microarray, Green fluorescence protein",
author = "Abhik Bandyopadhyay and Abdel Elkahloun and Baysa, {Sherrie Joy} and Long Wang and Luzhe Sun",
year = "2005",
month = "2",
language = "English (US)",
volume = "4",
pages = "168--174",
journal = "Cancer Biology and Therapy",
issn = "1538-4047",
publisher = "Landes Bioscience",
number = "2",

}

TY - JOUR

T1 - Development and gene expression profiling of a metastatic variant of the human breast cancer MDA-MB-435 cells

AU - Bandyopadhyay, Abhik

AU - Elkahloun, Abdel

AU - Baysa, Sherrie Joy

AU - Wang, Long

AU - Sun, Luzhe

PY - 2005/2

Y1 - 2005/2

N2 - We have developed a model system of late stage metastatic progression by isolating a highly malignant variant of human breast cancer cells from the parental MDA-MB-435 cell line. These cells, isolated from early lung metastasis, displayed increased anchorage independent growth in vitro and when transplanted ortho-topically into nude mice showed accelerated tumor growth rate and early lung spontaneous metastasis when compared to its parental counterpart. These cells, designated as MDA-MB-435-F-L, also showed intense wide spread early skeletal metastasis in vertebrae, mandible, femur, tibia and skull as detected by fluorescence imaging in an experimental bone metastasis model. Gene expression profiles from cDNA microarray showed up or downregulation of the expression of several significant genes regulating angiogenesis, apoptosis, ECM remodeling and metastasis in the MDA-MB-435-F-L cells in comparison to the parental cells. Among the up or downregulated genes, some have also been implicated in the survival of breast cancer patients. As such, the candidate genes selected in this breast cancer progression model system may serve as biomarkers of metastatic progression and also as potential tumor targets for breast cancer therapy.

AB - We have developed a model system of late stage metastatic progression by isolating a highly malignant variant of human breast cancer cells from the parental MDA-MB-435 cell line. These cells, isolated from early lung metastasis, displayed increased anchorage independent growth in vitro and when transplanted ortho-topically into nude mice showed accelerated tumor growth rate and early lung spontaneous metastasis when compared to its parental counterpart. These cells, designated as MDA-MB-435-F-L, also showed intense wide spread early skeletal metastasis in vertebrae, mandible, femur, tibia and skull as detected by fluorescence imaging in an experimental bone metastasis model. Gene expression profiles from cDNA microarray showed up or downregulation of the expression of several significant genes regulating angiogenesis, apoptosis, ECM remodeling and metastasis in the MDA-MB-435-F-L cells in comparison to the parental cells. Among the up or downregulated genes, some have also been implicated in the survival of breast cancer patients. As such, the candidate genes selected in this breast cancer progression model system may serve as biomarkers of metastatic progression and also as potential tumor targets for breast cancer therapy.

KW - Bone metastasis

KW - Breast cancer

KW - cDNA-microarray

KW - Green fluorescence protein

UR - http://www.scopus.com/inward/record.url?scp=25144454435&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=25144454435&partnerID=8YFLogxK

M3 - Article

C2 - 15655343

AN - SCOPUS:25144454435

VL - 4

SP - 168

EP - 174

JO - Cancer Biology and Therapy

JF - Cancer Biology and Therapy

SN - 1538-4047

IS - 2

ER -