Development and fertility in Caenorhabditis elegans clk-1 mutants depend upon transport of dietary coenzyme Q8 to mitochondria

Tanya Jonassen, Beth N. Marbois, Kym F. Faull, Catherine F. Clarke, Pamela L Larsen

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

The Caenorhabditis elegans clk-1 mutants lack coenzyme Q9 and instead accumulate the biosynthetic intermediate demethoxy-Q9 (DMQ9). clk-1 animals grow to reproductive adults, albeit slowly, if supplied with Q8-containing Escherichia coli. However, if Q is withdrawn from the diet, clk-1 animals either arrest development as young larvae or become sterile adults depending upon the stage at the time of the withdrawal. To understand this stage-dependent response to a Q-less diet, the quinone content was determined during development of wild-type animals. The quinone content varies in the different developmental stages in wild-type fed Q8-replete E. coli. The amounts peak at the second larval stage, which coincides with the stage of arrest of clk-1 larvae fed a Q-less diet from hatching. Levels of the endogenously synthesized DMQ9 are high in the clk-1(qm30)-arrested larvae and sterile adults fed Q-less food. Comparison of quinones from animals fed a Q-replete or a Q-less diet establishes that the Q8 present is assimilated from the E. coli. Furthermore, this E. coli-specific Q8 is present in mitochondria isolated from fertile clk-1(qm30) adults fed a Q-replete diet. These results suggest that the uptake and transport of dietary Q8 to mitochondria prevent the arrest and sterility phenotypes of clk-1 mutants and that DMQ is not functionally equivalent to Q.

Original languageEnglish (US)
Pages (from-to)45020-45027
Number of pages8
JournalJournal of Biological Chemistry
Volume277
Issue number47
DOIs
StatePublished - Nov 22 2002
Externally publishedYes

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Mitochondria
Caenorhabditis elegans
Nutrition
Fertility
Escherichia coli
Diet
Animals
Larva
Quinones
Wild Animals
Infertility
ubiquinone 8
Phenotype
Food

ASJC Scopus subject areas

  • Biochemistry

Cite this

Development and fertility in Caenorhabditis elegans clk-1 mutants depend upon transport of dietary coenzyme Q8 to mitochondria. / Jonassen, Tanya; Marbois, Beth N.; Faull, Kym F.; Clarke, Catherine F.; Larsen, Pamela L.

In: Journal of Biological Chemistry, Vol. 277, No. 47, 22.11.2002, p. 45020-45027.

Research output: Contribution to journalArticle

Jonassen, Tanya ; Marbois, Beth N. ; Faull, Kym F. ; Clarke, Catherine F. ; Larsen, Pamela L. / Development and fertility in Caenorhabditis elegans clk-1 mutants depend upon transport of dietary coenzyme Q8 to mitochondria. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 47. pp. 45020-45027.
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