Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing Aspergillosis

G. Marshall Lyon; Deborah Abdul-Ali; Juergen Loeffler; P. Lewis White; Brian Wickes; Monica L. Herrera; Barbara D. Alexander; Lindsey R. Baden; Cornelius Clancy; David Denning; M. Hong Nguyen; Michele Sugrue; L. Joseph Wheat; John R. Wingard; J. Peter Donnelly; Rosemary Barnes; Thomas F. Patterson; Angela M. Caliendo

doi:10.1128/JCM.00744-13

Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing Aspergillosis

G. Marshall Lyon
, Deborah Abdul-Ali
, Juergen Loeffler
, P. Lewis White
, Brian Wickes
, Monica L. Herrera
, Barbara D. Alexander
, Lindsey R. Baden
, Cornelius Clancy
, David Denning
, M. Hong Nguyen
, Michele Sugrue
, L. Joseph Wheat
, John R. Wingard
, J. Peter Donnelly
, Rosemary Barnes
, Thomas F. Patterson
, Angela M. Caliendo

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Twelve laboratories evaluated candidate material for an Aspergillus DNA calibrator. The DNA material was quantified using limiting-dilution analysis; the mean concentration was determined to be 1.73-1010 units/ml. The calibrator can be used to standardize aspergillosis diagnostic assays which detect and/or quantify nucleic acid.

Original language	English (US)
Pages (from-to)	2403-2405
Number of pages	3
Journal	Journal of clinical microbiology
Volume	51
Issue number	7
DOIs	https://doi.org/10.1128/JCM.00744-13
State	Published - Jul 2013

ASJC Scopus subject areas

Microbiology (medical)

Access to Document

10.1128/JCM.00744-13

Cite this

Lyon, G. M., Abdul-Ali, D., Loeffler, J., White, P. L., Wickes, B., Herrera, M. L., Alexander, B. D., Baden, L. R., Clancy, C., Denning, D., Nguyen, M. H., Sugrue, M., Wheat, L. J., Wingard, J. R., Donnelly, J. P., Barnes, R., Patterson, T. F., & Caliendo, A. M. (2013). Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing Aspergillosis. Journal of clinical microbiology, 51(7), 2403-2405. https://doi.org/10.1128/JCM.00744-13

Lyon, GM, Abdul-Ali, D, Loeffler, J, White, PL, Wickes, B, Herrera, ML, Alexander, BD, Baden, LR, Clancy, C, Denning, D, Nguyen, MH, Sugrue, M, Wheat, LJ, Wingard, JR, Donnelly, JP, Barnes, R, Patterson, TF & Caliendo, AM 2013, 'Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing Aspergillosis', Journal of clinical microbiology, vol. 51, no. 7, pp. 2403-2405. https://doi.org/10.1128/JCM.00744-13

@article{db98a75124ae48be91c096be513f48d1,

title = "Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing Aspergillosis",

abstract = "Twelve laboratories evaluated candidate material for an Aspergillus DNA calibrator. The DNA material was quantified using limiting-dilution analysis; the mean concentration was determined to be 1.73-1010 units/ml. The calibrator can be used to standardize aspergillosis diagnostic assays which detect and/or quantify nucleic acid.",

author = "Lyon, \{G. Marshall\} and Deborah Abdul-Ali and Juergen Loeffler and White, \{P. Lewis\} and Brian Wickes and Herrera, \{Monica L.\} and Alexander, \{Barbara D.\} and Baden, \{Lindsey R.\} and Cornelius Clancy and David Denning and Nguyen, \{M. Hong\} and Michele Sugrue and Wheat, \{L. Joseph\} and Wingard, \{John R.\} and Donnelly, \{J. Peter\} and Rosemary Barnes and Patterson, \{Thomas F.\} and Caliendo, \{Angela M.\}",

year = "2013",

month = jul,

doi = "10.1128/JCM.00744-13",

language = "English (US)",

volume = "51",

pages = "2403--2405",

journal = "Journal of clinical microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "7",

}

TY - JOUR

T1 - Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing Aspergillosis

AU - Lyon, G. Marshall

AU - Abdul-Ali, Deborah

AU - Loeffler, Juergen

AU - White, P. Lewis

AU - Wickes, Brian

AU - Herrera, Monica L.

AU - Alexander, Barbara D.

AU - Baden, Lindsey R.

AU - Clancy, Cornelius

AU - Denning, David

AU - Nguyen, M. Hong

AU - Sugrue, Michele

AU - Wheat, L. Joseph

AU - Wingard, John R.

AU - Donnelly, J. Peter

AU - Barnes, Rosemary

AU - Patterson, Thomas F.

AU - Caliendo, Angela M.

PY - 2013/7

Y1 - 2013/7

N2 - Twelve laboratories evaluated candidate material for an Aspergillus DNA calibrator. The DNA material was quantified using limiting-dilution analysis; the mean concentration was determined to be 1.73-1010 units/ml. The calibrator can be used to standardize aspergillosis diagnostic assays which detect and/or quantify nucleic acid.

AB - Twelve laboratories evaluated candidate material for an Aspergillus DNA calibrator. The DNA material was quantified using limiting-dilution analysis; the mean concentration was determined to be 1.73-1010 units/ml. The calibrator can be used to standardize aspergillosis diagnostic assays which detect and/or quantify nucleic acid.

UR - https://www.scopus.com/pages/publications/84879450059

UR - https://www.scopus.com/pages/publications/84879450059#tab=citedBy

U2 - 10.1128/JCM.00744-13

DO - 10.1128/JCM.00744-13

M3 - Article

C2 - 23616459

AN - SCOPUS:84879450059

SN - 0095-1137

VL - 51

SP - 2403

EP - 2405

JO - Journal of clinical microbiology

JF - Journal of clinical microbiology

IS - 7

ER -

Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing Aspergillosis

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this