Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing Aspergillosis

G. Marshall Lyon, Deborah Abdul-Ali, Juergen Loeffler, P. Lewis White, Brian L Wickes, Monica L. Herrera, Barbara D. Alexander, Lindsey R. Baden, Cornelius Clancy, David Denning, M. Hong Nguyen, Michele Sugrue, L. Joseph Wheat, John R. Wingard, J. Peter Donnelly, Rosemary Barnes, Thomas F Patterson, Angela M. Caliendo

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Twelve laboratories evaluated candidate material for an Aspergillus DNA calibrator. The DNA material was quantified using limiting-dilution analysis; the mean concentration was determined to be 1.73-1010 units/ml. The calibrator can be used to standardize aspergillosis diagnostic assays which detect and/or quantify nucleic acid.

Original languageEnglish (US)
Pages (from-to)2403-2405
Number of pages3
JournalJournal of Clinical Microbiology
Volume51
Issue number7
DOIs
StatePublished - 2013

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Aspergillosis
Nucleic Acids
DNA
Aspergillus

ASJC Scopus subject areas

  • Microbiology (medical)
  • Medicine(all)

Cite this

Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing Aspergillosis. / Lyon, G. Marshall; Abdul-Ali, Deborah; Loeffler, Juergen; White, P. Lewis; Wickes, Brian L; Herrera, Monica L.; Alexander, Barbara D.; Baden, Lindsey R.; Clancy, Cornelius; Denning, David; Nguyen, M. Hong; Sugrue, Michele; Wheat, L. Joseph; Wingard, John R.; Donnelly, J. Peter; Barnes, Rosemary; Patterson, Thomas F; Caliendo, Angela M.

In: Journal of Clinical Microbiology, Vol. 51, No. 7, 2013, p. 2403-2405.

Research output: Contribution to journalArticle

Lyon, GM, Abdul-Ali, D, Loeffler, J, White, PL, Wickes, BL, Herrera, ML, Alexander, BD, Baden, LR, Clancy, C, Denning, D, Nguyen, MH, Sugrue, M, Wheat, LJ, Wingard, JR, Donnelly, JP, Barnes, R, Patterson, TF & Caliendo, AM 2013, 'Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing Aspergillosis', Journal of Clinical Microbiology, vol. 51, no. 7, pp. 2403-2405. https://doi.org/10.1128/JCM.00744-13
Lyon, G. Marshall ; Abdul-Ali, Deborah ; Loeffler, Juergen ; White, P. Lewis ; Wickes, Brian L ; Herrera, Monica L. ; Alexander, Barbara D. ; Baden, Lindsey R. ; Clancy, Cornelius ; Denning, David ; Nguyen, M. Hong ; Sugrue, Michele ; Wheat, L. Joseph ; Wingard, John R. ; Donnelly, J. Peter ; Barnes, Rosemary ; Patterson, Thomas F ; Caliendo, Angela M. / Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing Aspergillosis. In: Journal of Clinical Microbiology. 2013 ; Vol. 51, No. 7. pp. 2403-2405.
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AU - Wickes, Brian L

AU - Herrera, Monica L.

AU - Alexander, Barbara D.

AU - Baden, Lindsey R.

AU - Clancy, Cornelius

AU - Denning, David

AU - Nguyen, M. Hong

AU - Sugrue, Michele

AU - Wheat, L. Joseph

AU - Wingard, John R.

AU - Donnelly, J. Peter

AU - Barnes, Rosemary

AU - Patterson, Thomas F

AU - Caliendo, Angela M.

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