Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing Aspergillosis

G. Marshall Lyon, Deborah Abdul-Ali, Juergen Loeffler, P. Lewis White, Brian Wickes, Monica L. Herrera, Barbara D. Alexander, Lindsey R. Baden, Cornelius Clancy, David Denning, M. Hong Nguyen, Michele Sugrue, L. Joseph Wheat, John R. Wingard, J. Peter Donnelly, Rosemary Barnes, Thomas F. Patterson, Angela M. Caliendo

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Twelve laboratories evaluated candidate material for an Aspergillus DNA calibrator. The DNA material was quantified using limiting-dilution analysis; the mean concentration was determined to be 1.73-1010 units/ml. The calibrator can be used to standardize aspergillosis diagnostic assays which detect and/or quantify nucleic acid.

Original languageEnglish (US)
Pages (from-to)2403-2405
Number of pages3
JournalJournal of clinical microbiology
Volume51
Issue number7
DOIs
StatePublished - Jul 2013

ASJC Scopus subject areas

  • Microbiology (medical)

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    Lyon, G. M., Abdul-Ali, D., Loeffler, J., White, P. L., Wickes, B., Herrera, M. L., Alexander, B. D., Baden, L. R., Clancy, C., Denning, D., Nguyen, M. H., Sugrue, M., Wheat, L. J., Wingard, J. R., Donnelly, J. P., Barnes, R., Patterson, T. F., & Caliendo, A. M. (2013). Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing Aspergillosis. Journal of clinical microbiology, 51(7), 2403-2405. https://doi.org/10.1128/JCM.00744-13