Development and Characterization of Human Ependymoma Xenograft HxBr51

Janet A. Houghton, Peter J Houghton, Larry E. Kun

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We describe the successful heterotransplantation of a human ependymoma in CBA/CaJ mice immune deprived by infant thymectomy and whole-body irradiation. The xenograft, HxBr5, was established from a fourth ventricular ependymoma, locally recurrent in an 11-yr-old girl who had been treated with radiation therapy to the posterior fossa. HxBr5 retains histological and ultrastructural fidelity to the tumor from which it was derived as does the DNA content, as confirmed by flow cytometric analysis. The karyotype of the xenograft, which is pseudodiploid and exhibits trisomy lq and deletion of 1p, is the first human ependymoma banded karyotype to be reported. Growth rates of the xenograft tumors are similar to the primary tumor as clinically observed with a doubling time of approximately 42 days. Cell kinetic parameters indicate that this slow-growing tumor has a relatively high growth fraction of 70.8% with a high cell loss of approximately 91%. We anticipate that HxBr5 may be useful as one component of a more complex model for studying the biology and differentiation of human ependymoma.

Original languageEnglish (US)
Pages (from-to)499-504
Number of pages6
JournalCancer Research
Volume47
Issue number2
StatePublished - Jan 1 1987
Externally publishedYes

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Ependymoma
Human Development
Heterografts
Karyotype
Neoplasms
Thymectomy
Inbred CBA Mouse
Whole-Body Irradiation
Trisomy
Growth
Radiotherapy
DNA

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Development and Characterization of Human Ependymoma Xenograft HxBr51. / Houghton, Janet A.; Houghton, Peter J; Kun, Larry E.

In: Cancer Research, Vol. 47, No. 2, 01.01.1987, p. 499-504.

Research output: Contribution to journalArticle

Houghton, Janet A. ; Houghton, Peter J ; Kun, Larry E. / Development and Characterization of Human Ependymoma Xenograft HxBr51. In: Cancer Research. 1987 ; Vol. 47, No. 2. pp. 499-504.
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