Determination of voriconazole in aqueous humor by liquid chromatography-electrospray ionization-mass spectrometry

Lei Zhou, Randolph D. Glickman, Nancy Chen, William E. Sponsel, John R. Graybill, Kwok Wai Lam

Research output: Contribution to journalArticle

69 Scopus citations

Abstract

A novel method based on liquid chromatography-mass spectrometry with electrospray ionization (LC-MS) has been developed for analysis of voriconazole in aqueous humor. The separation was achieved on a reversed-phase C18 column eluted by 70% acetonitrile-30% water-0.01% TFA. The correlation between the concentration of voriconazole to peak area was linear (r2=0.9990) between 0.04 and 60 ng, with a coefficient of variance of less than 3%. Limit of quantitation (LOQ) was estimated to be 5 ng/ml voriconazole with an injection volume of 2 μl of aqueous humor. Both intra-day and inter-day imprecision were less than 3% over the whole analytical range. Parallel analyses of voriconazole samples by LC-MS and by high-performance liquid chromatography (HPLC)-UV showed that the two methods were highly correlated (r2=0.9985). LC-MS was used to the determine voriconazole levels achieved in the aqueous humor of the rabbit eye, following topical application of 5 or 10 μg voriconazole in the form of eyedrops for 11 days b.i.d. The lower dosage produced an aqueous humor concentration of 7.29±5.84 μg/ml, while the higher dosage produced a concentration of 14.56±12.90 μg/ml.

Original languageEnglish (US)
Pages (from-to)213-220
Number of pages8
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume776
Issue number2
DOIs
StatePublished - Sep 5 2002

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Keywords

  • Antifungal
  • Aqueous humor
  • Voriconazole

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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