TY - JOUR
T1 - Determination of the epitope of an inhibitory antibody to proliferating cell nuclear antigen
AU - Roos, Göran
AU - Jiang, Yunguan
AU - Landberg, Göran
AU - Nielsen, Niels H.
AU - Zhang, Peng
AU - Lee, Marietta Y.W.T.
N1 - Funding Information:
This study was supported by grants from the Swedish Cancer Society, The Medical Faculty, UmeaÊ University, the Lions Cancer Research Foundation in UmeaÊ, a special grant from VaÈsterbotten County Council, and NIH Grant GM31973 to M. Y. W. T. Lee.
PY - 1996/7/10
Y1 - 1996/7/10
N2 - Proliferating cell nuclear antigen (PCNA) is an essential component for the normal processive DNA synthesis by DNA polymerase δ and is also required for DNA excision repair. Human PCNA autoautisera has been shown to inhibit the function of PCNA in vitro, in contrast to induced antibodies. A monoclonal IgG2 PCNA antibody, 74B1, that effectively inhibited DNA replication in vitro was identified. The inhibitory effect was dose dependent and using synthetic overlapping peptides of PCNA the 74B1 epitope was mapped to aa 121-135, a region of the PCNA protein containing the interdomain connector implicated in intermolecular interactions. Interestingly, a neighboring and partly overlapping peptide, aa 111-125, contains an immunodominant region recognized by a number of monoclonal PCNA antibodies with no inhibitory effect on DNA synthesis. The 74B1 antibody is a potentially useful antibody in future studies of PCNA and its interaction in complexes associated with cell cycle progress, DNA replication, and DNA repair.
AB - Proliferating cell nuclear antigen (PCNA) is an essential component for the normal processive DNA synthesis by DNA polymerase δ and is also required for DNA excision repair. Human PCNA autoautisera has been shown to inhibit the function of PCNA in vitro, in contrast to induced antibodies. A monoclonal IgG2 PCNA antibody, 74B1, that effectively inhibited DNA replication in vitro was identified. The inhibitory effect was dose dependent and using synthetic overlapping peptides of PCNA the 74B1 epitope was mapped to aa 121-135, a region of the PCNA protein containing the interdomain connector implicated in intermolecular interactions. Interestingly, a neighboring and partly overlapping peptide, aa 111-125, contains an immunodominant region recognized by a number of monoclonal PCNA antibodies with no inhibitory effect on DNA synthesis. The 74B1 antibody is a potentially useful antibody in future studies of PCNA and its interaction in complexes associated with cell cycle progress, DNA replication, and DNA repair.
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U2 - 10.1006/excr.1996.0220
DO - 10.1006/excr.1996.0220
M3 - Article
C2 - 8660957
AN - SCOPUS:0005138259
SN - 0014-4827
VL - 226
SP - 208
EP - 213
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -