Determination of anandamide and other fatty acyl ethanolamides in human serum by electrospray tandem mass spectrometry

Daniela Schreiber, Stephanie Harlfinger, Brit M. Nolden, Christoph W. Gerth, Ulrich Jaehde, Edgar Schömig, Joachim Klosterkötter, Andrea Giuffrida, Giuseppe Astarita, Daniele Piomelli, F. Markus Leweke

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

We developed a new selective liquid chromatography-electrospray ionization-tandem mass spectrometry method for the identification and quantification of anandamide (AEA), an endogenous cannabinoid receptor ligand, and other bioactive fatty acid ethanolamides (FAEs) in biological samples. Detection limit (0.025 pmol for AEA and 0.1 pmol for palmitoylethanolamide (PEA) and oleoylethanolamide (OEA)) and quantification limit (0.2 pmol for AEA and 0.4 pmol for OEA and PEA) were in the high fmol to low pmol range for all analytes. Linear correlations (r2 = 0.99) were observed in the calibration curves for standard AEA over the range of 0.025-25 pmol and for standard PEA and OEA over the range of 0.1-500 pmol. This method provides a time-saving and sensitive alternative to existing methods for the analysis of FAEs in biological samples.

Original languageEnglish (US)
Pages (from-to)162-168
Number of pages7
JournalAnalytical Biochemistry
Volume361
Issue number2
DOIs
StatePublished - Feb 15 2007

Keywords

  • Anandamide
  • Fatty acid ethanolamides
  • Human
  • Liquid chromatography
  • Tandem mass spectrometry

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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