Determination of a particle's radius by two-dimensional agarose gel electrophoresis

Philip Serwer, Shirley J. Hayes, Gary A. Griess

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Electrophoresis in an agarose gel dilute enough to be almost nonretarding, followed by electrophoresis in an orthogonal direction into a more concentrated agarose gel, has been developed as a procedure to determine the radius of spherical particles. Unlike procedures of unidirectional electrophoresis in a single gel, the above procedure can be used to compare the radii of particles that differ in solid-support-free electrophoretic mobility. Accuracy of 0.3 nm has been achieved with particles 30 nm in radius. It was found that the apparent radius of the spherical capsid of bacteriophage P22 decreased by 3% during elevated temperature-induced ejection of DNA from the capsid. Though originally designed for use with multimolecular paricles, the procedure described here should also be useful with monomolecular particles.

Original languageEnglish (US)
Pages (from-to)339-345
Number of pages7
JournalAnalytical Biochemistry
Volume152
Issue number2
DOIs
Publication statusPublished - Feb 1 1986

    Fingerprint

Keywords

  • gel electrophoresis,two dimensional
  • multiple embedded gels
  • spheres,radius determination
  • spherical viruses
  • viral capsids

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this