Deteriorating collagen synthesis and cell ultrastructure accompanying senescence of human normal and Werner's syndrome fibroblast cell strains

Joseph W Basler, John D. David, Paul F. Agris

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Ultrastructural changes that accompanied senescence, in vitro, of six normal fibroblast cell strains were found to correspond to the morphological characteristics of a Werner's syndrome progeroid cell strain: greatly increased size of midnuclear diameters; heavy vacuolation; maturation of predominantly primary to secondary lysosomes; increase in multilobed nuclei; dilation of mitochondrial cristae; dilation of rough endoplasmic reticulum and the filling of intermembrane space with electron-dense particles presumably protein; and vacuolar accumulation of fibrous material which was later discharged into the medium. This fibrous material was produced only by late-passage fibroblasts and Werner's syndrome fibroblasts and had an ultrastructure dissimilar from that of normal collagen fibers produced by exponentially-growing cultures. The fibrous debris had a high subunit molecular weight (approx. 200000 D), an amino acid composition that included hydroxyproline, hydroxylysine, and relatively large amounts of proline and glycine, and was degraded by collagenase. This protein is believed to be a form of procollagen produced by either incorrect maturation of mRNA or incomplete processing of procollagen.

Original languageEnglish (US)
Pages (from-to)73-84
Number of pages12
JournalExperimental Cell Research
Volume118
Issue number1
DOIs
StatePublished - 1979
Externally publishedYes

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Werner Syndrome
Procollagen
Collagen
Fibroblasts
Dilatation
Hydroxylysine
Rough Endoplasmic Reticulum
Hydroxyproline
Collagenases
Lysosomes
Proline
Glycine
Proteins
Molecular Weight
Electrons
Amino Acids
Messenger RNA

ASJC Scopus subject areas

  • Cell Biology

Cite this

Deteriorating collagen synthesis and cell ultrastructure accompanying senescence of human normal and Werner's syndrome fibroblast cell strains. / Basler, Joseph W; David, John D.; Agris, Paul F.

In: Experimental Cell Research, Vol. 118, No. 1, 1979, p. 73-84.

Research output: Contribution to journalArticle

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